지충이 추출물의 Lipase 및 α-Amylase 저해활성

Abstract

Diabetes and obesity are the most serious and chronic disease of the general population over the world. One therapeutic approach for the prevention of diabetes and obesity has being made to investigate potential inhibitor against lipase and α-amylase from natural sources. This study was performed to investigate the inhibitory activity against lipase and α-amylase to elucidate the possible use of seaweed extracts as an anti-obese and anti-diabetic agent. So, we measured lipase and α-amylase inhibitory activity from 21 species of seaweed extracts. As a result, Enteromorpha intenstinalis, Porphya yezoensis and Sargassum thunbergii ethanol extract showed 58, 49 and 37% lipase inhibitory activity and Sargassum thunbergii, Sargassum siliguastrum ethanol extract showed 69 and 60% α-amylase inhibitory activity. These results suggest that Sargassum thunbergii(ST), a brown algae, evidenced prominent inhibitory activity against lipase and α-amylase. In the inhibitory test, ST ethanol extract showed 37, 19 and 16% lipase inhibitory activity, and the α-amylase inhibitory activity of 69, 54 and 33% at concentrations of 5, 2.5 and 1 mg/mL. In order to study the changes in enzyme inhibitory activity by heat, pH and γ-irradiation, ST ethanol extract was carried out by heat, pH and γ-irradiation(3-20 kGy) test. In the heat treatment, all treatments were increased than the untreated. In the pH treatment, stable activity showed range to pH 4-8. And ST extract was no significant changes by γ-irradiation. ST ethanol extract was separated into five different partition layers, n-hexane, chloroform, ethyl acetate, n-butanol and water layer, by solvent extraction. As a result of enzyme inhibitory activity for each obtained fractions, out of four different partition layers excepted water layer showed higher activity than ethanol extract. Especially, chloroform fraction exhibited the strongest lipase inhibitory activity of 63% at a concentration of 2.5 mg/mL. α-Amylase inhibitory activity showed also higher value than acarbose(positive control) in chloroform fraction. Therefore can be used ST extract as the natural inhibitor of anti-diabetic and anti-obesity.