참가리비 (Patinopecten yessoensis )의 난 성숙단계에 따라 다르게 발현된 4개 유전자 동정

Abstract

Despite great commercial interest, relatively little has been described about molecular mechanism of bivalve reproduction. We investigated genes involved in maturation of the ezo scallop, Patinopecten yessoensis. Histological analysis revealed that maturation of ovary begins in February and spawning period is from April to June which is similar to the previous study in the East Sea. As result of combination analysis of differential display RT-PCR (DDRT-PCR) and histological examination, Vitellogenin (Vg), pancreatic lipase related protein (PLRP), Ferritin (Ft) and ADT/ATP carrier protein (ACC) were identified as differently expressed genes in maturating ovary. End-point RT-PCR results showed that Vg and PLRP are ovary-specific genes whereas Ft and ACC are expressed ubiquitously suggesting that Vg and PLRP are good molecular markers for ovarian development and sex determination in bivalves. Quantitative PCR results revealed that both Vg and PRLP were expressed highest during growth stage and they appear to play a major role in oocyte maturation. This result indicates that these two genes are also important molecular markers for maturating oocytes in bivalves or other mollusks. On the contrary, Expression of Ft was highest after spawning stage, which suggests that up-regulation may be involved in spawning and inactive stages in which the scallops recover from spawning. In addition, high level of the mitochondrial gene, ACC, may play a role in energy metabolism in maturating oocytes. The isolation and molecular studies of these key genes will expand our knowledge of the physiological changes from various exogenous factors including temperature, salinity, pH, even or numerous endocrine disrupting chemicals (EDCs) during reproduction period. In addition, further study of these genes implicates various industrial applications including the stable seed production, increased food quality, or economic aquaculture system.