Anti-inflammatory effect of peptides isolated from Seahorse, Hippocampus Kuda Bleeler, on human osteoblastic cells (MG-63) and chondrocytic cells (SW-1353)

Abstract

During osteoarthritis, chondrocyte or osteoblast differentiation can be affected on extracellular matrix. Although underlying mechanisms have not been elucidated, evidence points out the participation of the nitric oxide (NO) and matrix metalloproteinases (MMPs). In the present study, two peptides having anti-inflammation properties were isolated from seahorse protein, Hippocampus Kuda Bleeler which a marine teleost fish. Seahorse protein was hydrolyzed using several enzymes. Among hydrolysates, Pronase E hydrolysate exhibited the highest inflammation inhibitory activity. In order to purity, the amino acid sequence of the two peptide hydrolysate was determined to having potent anti-inflammation properties. These two peptides promoted osteoblastic differentiation human osteoblastic and chondrocytic cells, respectively. Furthermore, those peptides inhibited mRNA gene and protein levels of MMP-1, MMP-3, MMP-13, inflammatory response of iNOS and COX-2. In addition, it was observed that the peptides inhibited phosphorylation of MAPK pathway in human osteoblastic and chondrocytic cells. This may explain some of the mechanisms whereby the peptides exerts its positive effect on osteoarthritis changes.