상피세포 성장인자와 콜라겐 결합능이 있는 기능성 단백질의 개발

Abstract

Human epidermal growth factor (hEGF) is a polypeptide with 53 amino acids in mammalian species, a one of the important autocrine/paracrine factor of human body and applied in pharmaceutical and cosmetics industries because of its biochemical multifunction. However, low level of production from natural sources, limited target specificity and short half-life of this useful factor make problems as therapeutic agent. To avoid these troubles, we constructed the fusion hEGF proteins with collagen-binding domain (VMCBD) from Vibrio mimicusmetalloprotease. This collagen-binding domain, peptide with 33 amino acids, has shown previously that essential for the collagen binding activity of V. mimicus metalloprotease and fused at the C-terminal of hEGF protein and the fusion proteins were expressed in Escherichia coli. Treatment of C-terminal fused hEGF protein was revealed more promoted growth number of human/A431 cells than control hEGF. These results suggest that VMCBD fused recombinant hEGF proteins can be used for the epidermal cell healing agent.