Desmthylanhydroicaritin의 인간 뇌교종 암세포 에서의 항암 효능 확인
- Alternative Title
- Anti-cancer Effects of Desmethylanhydroicaritin on U87MG Human Glioblastoma Cells
- Abstract
- We investigated anti-cancer effects of desmethylanhydroicaritin (DMAI), a major compound of the Chinese herbal medicine Epimedium, on Human cancer cell lines, including glioblastoma cancer U87MG cells, human lung cancer A-549 cells, human colorectal cancer HT-29 cells and human gastric cancer AGS cells. Among the all these cancer cell lines, U87MG cells were showed hight sensitive induced cell death by DMAI. DMAI showed cytotoxicity effects on U87MG cells at the concentration of 30 to 40 μM, however did not had any effects on HEK-293 cells. DMAI induced anti-proliferation effects via Ras/Raf/MEK/ERK MAPkinase, PI3K/Akt/mTOR signal pathway and G2/M phase cell cycle arrest by down-regulating p-Cdc 25 C, p-Cdc 2 and cyclin B 1. DMAI led to morphological change and inhibition of filapodia formation through regulation of Rac 1 and Cdc 42. In addition, migration and invasion of U87MG cells were inhibited by DMAI via down-regulation of matrix metalloproteinase (MMP)-2 and -9 expressions and activity. Our results suggest that DMAI has a potential as therapeutic agent against glioblastoma cells.
- Issued Date
- 2015
- Awarded Date
- 2015. 2
- Type
- Dissertation
- Publisher
- 부경대학교
- Alternative Author(s)
- Chang Won Kang
- Affiliation
- 부경대학교
- Department
- 대학원 미생물학과
- Advisor
- 김군도
- Table Of Contents
- 1. INTRODUCTION 1
2. MATERIAL AND METHODS 3
2.1 Isolation of Desmethylanhydroicaritin 3
2.2 Cell culture 3
2.3 Cell viability assay 4
2.4 Protein extraction and Western blot analysis 5
2.5 Wound healing assay 6
2.6 Transwell invasion assay 6
2.7 Immunofluorescence staining 7
2.8 FACS analysis 8
2.9 Gelatin zymography 8
2.10 Statistical analysis 9
3. RESULTS 10
3.1 DMAI induces anti-proliferation in glioblstoma 10
3.2 DMAI induces apoptosis in glioblastoma 12
3.3 DMAI inhibits Raf/MEK/ERK MAPK pathway in U87MG cells 14
3.4 DMAI inhibits the PI3K/Akt/mTOR/p70S6K pathway in U87MG cells 16
3.5 DMAI induces G2/M cell cycle arrest on U87MG cells 18
3.6 DMAI inhibits the formation filapodia in glioblastoma cells through down-regulation of Cdc 42 and Rac 1 expression 20
3.7 DMAI inhibits invasion and migration of U87MG cells 22
4. DISCUSSION 26
5. 국문초록 32
6. Acknowledgement 33
7. REFERENCES 34
- Degree
- Master
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