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Methyl Mercaptan에 대한 식물 유래 폴리페놀 화합물의 탈취 효과

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Abstract
This study was conducted to elucidate deodorizing effect of polyphenolic phytochemicals against methyl mercaptan. Eckol, dieckol, catechol, catechin, phloroglucinol, chlorogenic acid, epigallocatechin gallate (EGCG), epicatehcin gallate (ECG), epigallocatehcin (EGC), caffeic acid and tannic acid were used as polyphenolic phytochemicals. The deodorizing activity against methyl mercaptan was represented as IC50 (50% inhibitory concentration). methyl mercaptan was determined by GC-FPD. Dieckol (72.2±9.9 nM), one kind of phlorotannin, indicated the highest deodorizing activity against methyl mercaptan than sodium copper chlorophyllin (835.70 ± 24.36 nM) which known as a commercial deodorant. The high antimicrobial activity against Streptococcus mutans was observed in catechol (30.0±0.0 mm) by paper disc. Also, polyphenolic compounds such as catechol, EGC, eckol and dieckol have a high antimicrobial activity in the the minimun inhibitory concentration (MIC) in 0.25 mg/mL concentration. Inhibitory activity of glucosyltransferase (GTase) was measured depending on the concentration. Among them, eckol (98.6%) exhibited the highest inhibitory activity in 1 mg/mL concentration.
In conclusion, this study confirmed that polyphenolic phytochemicals have the deodoring activity against methyl mercaptan as well as antimicrobial activity on S. mutans.
Author(s)
김연
Issued Date
2015
Awarded Date
2015. 2
Type
Dissertation
Publisher
부경대학교
URI
https://repository.pknu.ac.kr:8443/handle/2021.oak/11887
http://pknu.dcollection.net/jsp/common/DcLoOrgPer.jsp?sItemId=000001967506
Alternative Author(s)
Kim yeon
Affiliation
부경대학교 일반대학원 식품공학과
Department
대학원 식품공학과
Advisor
김선봉
Table Of Contents
Ⅰ. 서 론 1
Ⅱ. 재료 및 방법 5
1. 실험재료 5
1.1 시료 5
1.2 시약 5
1.3 균주 6
1.4 배양 6
2. 실험방법 7
2.1 대황으로부터 eckol 및 dieckol 추출 및 분획 7
2.2 Methyl mercaptan 억제활성 7
2.2.1 Methyl mercaptan 표준 용액 제조 7
2.2.2 Methyl mercaptan 억제활성 측정 8
2.2.3 Gas chromatography 분석 조건 9
2.3 항균 활성 측정 9
2.3.1 한천 확산법 (Paper disc method) 9
2.3.2 최소 발육 억제 농도 측정 (MIC) 10
2.4 Glucosyltransferase 효소 저해 활성 11
2.4.1 GTase 조효소액 조제 11
2.4.2 GTase 기질 용액 조제 11
2.4.3 GTase 효소 저해 활성 측정 11
2.5 통계분석 12
Ⅲ. 결과 및 고찰 13
3.1 폴리페놀 화합물의 Methyl mercaptan 억제 활성 13
3.2 항균 활성 27
3.3 Glucosyltransferase 효소 저해 활성 31
Ⅳ. 요약 35
Ⅴ. 참고문헌 37
Degree
Master
Appears in Collections:
대학원 > 식품공학과
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