Protective effect of Non-replicating Baculovirus Vaccine in Artificial infection of Macrobrachium nipponense and Litopenaeus vannamei with White spot syndrome virus (WSSV)

Abstract

White spot syndrome virus (WSSV) causes the high mortality in shrimp farms, showing 100% mortality within 15 days. There have been many trials for the prevention of this fatal disease but there is no commercial vaccine available yet. In this study have developed and tested the efficacy of non-replicative recombinant baculovirus vaccine. The vaccine was constructed to express the vp28 and vp19 gene by the polyhedrin promoter and WSSV ie1 promoter, respectively. In the vaccine trial with 6 groups of vaccine, using Macrobrachium nipponense, not vaccinated group showed 100% cumulative mortality. In contrast, vaccinated group showed 10.2-33% cumulative mortality. Especially, the vaccine, Ac-VP28-ie1VP19+STF2, containing the WSSV VP28, VP19 genes and a flagellin gene of Salmonella was most effective. The vaccine (Ac-VP28-ie1VP19+STF2) was most effective vaccine in the trial with M. nipponense. After that, it was tested using Litopenaeus vannamei, was shown 22% cumulative mortality. It was shown that protective effects about WSSV infection, compared to 100% cumulative mortality in the non-vaccinated group.