The correlation between histopathological findings of liver and blood biochemical parameters in black rock fish, Sebastes schlegeli

Abstract

This study was carred out to examine the correlation among the blood biochemical parameters and the histological health in Korean black rock fish (Sebastes schlegeli).Fish mixed sex averaging 103.01±2.79g (mean ± SD) obtained from the farm in Tong-young city in Kyonganm province of korea, and acclimated for one week.Then, fish were separated into three groups(normal liver , abnormal(hypertrophic and fatty liver) and atrophic liver). Glass aquariums with external biofilters and artificial heaters were used. Fish were euthanized,blood samples were taken from each fish, blood were analyzed and measured some parameters such as Ht, Hb, TP, GOT and GPT. Fish were dissected at three time intervals. Tissues were fixed, re-fixed, processed, embedded with paraffin, sectioned and stained with hematoxylin& eosin for light microcopy. Morphological changes obsereved occurred were explained semi-quantitatively using indices. Significant differences (p<0.05) of indices were found between different liver alterations groups. Livers with normal yellow color were observed from normal livers and atrophic liver groups, respectively. and whitish-yellow color were observed from hypertrophic and fatty liver. Normal hepatocytes with circular, conspicuous and centrally located nuclei were found from normal livers group. Hypertrophic and fatty change were observed with large size hepatocytes and nuclei dislocated to the periphery of the cell by large sized lipid vacuoles. Atrophic hepatocytes were found with minute lipid vacuoles and inconspicuous nuclei. LRI and HSI indices were shown significant different between different liver alterations and giver real fact about health status for fish, while GOT were given opposite result in some liver alterations that didn't give us the real information about health fish. Hence, the results in this study suggested that there was no obvious correlation between blood biochemical parameters and histological health of liver.