거대녹조류 창자파래로부터 환원당 생산 및 항산화 활성 평가

Abstract

Abstract In chapter 1, to evaluate the efficacy of marine macro-algae Enteromorpha intestinalis as a potential bioenergy resource, the effects of reaction conditions (solid-to-liquid ratio, reaction temperature, and reaction time) on sugars produced by a combined process of hydrothermal and enzymatic hydrolysis were investigated. As a result of the hydrothermal hydrolysis, a 7.3 g/L (8% yield) total reducing sugar was obtained under conditions including solid-to-liquid ratio of 1:10, reaction temperature of 170°C, and reaction time of 60 min. By subsequent (post-hydrothermal) enzymatic hydrolysis of samples treated at 170°C for 30 min, a 20.1 g/L (22% yield) was achieved. In chapter 2, to evaluate antioxidant activities of E. intestinalis, three solvents (hexane, chloroform and methanol) were applied to obtain extracts. The extraction yields are hexane (A) 1.11%, chloroform (B) 0.94%, and methanol (C) 8.2%. Also, the contents of total phenolic compounds of extract A, B, C are 4.03%, 8.15%, and 2.33%, respectively. In the results of antioxidant activity, extract A and B present the higher DPPH activity than that of control (BHA and ascorbic acid). The DPPH radical scavenging activity and SOD-like activity are ordered as B>C>A. However, extract C show high value in ferric reducing antioxidant power assay. In nitrite scavenging activity, extract A is relatively higher than others. However, the antioxidant activities are lower than that of controls (ascorbic acid and BHA). The antioxidant activities are presented the increasing pattern of increasing by the increasing of extract concentration.