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다시마 분말에서 생산된 생리 활성 물질

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Alternative Title
Bioactive Compounds Produced from Laminiaria Japonica Powder
Abstract
최근 몇 년 동안 산업 공정이나 여러 요인들로 인해 해조류의 폐기량이 늘어나고 있다. 따라서, 산업 공정 과정을 통해 발생되는 해조 폐기물들의 효과적인 처리 방안이 요구 된다. 폐 해조류 중 갈조류는 laminarine과 alginate 등 다당류를 포함 하고 있다. 갈조류에는 antioxidant와 antibacterial의 기능이 있다고 알려져 있다. 본 연구에서는 Laminaria japonica 분말을 분해하여 그 기능의 능력을 알아보았다. 하지만 Laminaria japonica 분말은 용해도가 낮기 때문에 균들이 잘 분해 하지 못한다. 따라서 용해도를 증가시키기 위해서 pre-treatment 공정을 선택하였다. 이 공정은 물리적인 방법과 화학적은 방법을 사용하였다. 그 결과 용해도는 58%에서 94%로 증가하였다. Antioxidant 결과로는 reducing power는 0.263 g/L, Hydroxyl radical scavenging activity 는 99%, DPPH radical scavenging activity는 60%, 그리고 ABTS radical scavenging activity는 90%로 나왔다. 본 연구 결과를 통해서 Microbacterium oxydans을 이용하여 효과적으로 폐갈조류룰 분해하여 생리활성물질이나 부가 가치 높은 상품 생산 등으로 재활용하여 효과가 있을 것으로 사료 된다.
During recent years, the amount of seaweed waste has increased due to its culturing as an industrial resource and a depolluting plant for cleaning inland sea area, and eutrophication of seawater. The efficient treatment of seaweed waste is deemed necessary nowadays. Brown seaweed contains polysaccharides such as laminarin and alginate. brown seaweed is known as having antioxdant and antibacterial activitiy. In this study, we used Laminiaria japonica powder and some bacteria which have potential to degrade it. Because of its low solubility, it is difficult for bacteria to degrade brown seaweeds for producing sugars. So, for increasing solubility of the powder, pre-treatment was chosen. This procedure was divided two parts, physical and chemical treatment. As a result, the solubility was increased to 94% compared with its initial solubility (58%). Reducing power was 0.263 g/L. Hydroxyl radical scavenging activity was 99%. DPPH radical scavenging activity was 60%. ABTS radical scavenging activity was 90%.
Author(s)
강다솜
Issued Date
2015
Awarded Date
2015. 2
Type
Dissertation
Publisher
부경대학교
URI
https://repository.pknu.ac.kr:8443/handle/2021.oak/12001
http://pknu.dcollection.net/jsp/common/DcLoOrgPer.jsp?sItemId=000001967620
Alternative Author(s)
Kang Da Som
Affiliation
생물공학과
Department
대학원 생물공학과
Advisor
김중균
Table Of Contents
Ⅰ. INTRODUCTION-1

Ⅱ. MATERIALS AND METHODS-5
1. Useful microorganisms-5
2. Biodegradation of Laminaria japonica.-5
3. Determination of antioxidant activity-7
3.1 DPPH (2, 2- diphenyl - 1 - picrylhydrazyl) radical scavenging activity assay-7
3.2 ABTS radical cation decolorization assay-8
3.3 Hydroxyl radical scavenging activity assay-9
3.4 Reducing power assay-10
4. Analytical methods-11

Ⅲ. RESULTS AND DISCUSSION-13
1. Biodegradation of Laminaria japonica.-13
2. Biological functions of the biodegraded culture broths of Laminaria japonica.-20
2.1 Determination of antioxidant activity-20
2.1.1. DPPH Radical Scavenging Activity-20
2.1.2 ABTS Radical Cation Decolorization Assay-22
2.1.3 Hydroxyl Radical Scavenging Activity Assay-24
2.1.4 Reducing Power Assay-26

Ⅳ. CONCLUSION-28

Ⅴ. ACKNOWLEDGEMENTS-29

Ⅵ. REFERENCES-32
Degree
Master
Appears in Collections:
대학원 > 생물공학과
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