동물 실험을 통한 미역 추출물의 기억력 증강 효과

Abstract

In previous study, the effect of different seaweed extracts in growing of neuronal cell has been reported. This finding can be further supported by in vivo mice experiment. Therefore in this study we have evaluated memory of mice through Passive avoidance test (evaluate emotional memory),Morris water maze (spatial memory can be defined), and Radial arm maze (Working memory can be checked in). Juvenile C57BL and mature C57BL were used. There are three different concentration of extract groups. To prepare the extract, seaweed powder ethanolic extract (95%) with the ratio 50:1 was dried in vacuum and nitrogen dryer. In Passive avoidance test, box with two sides, dark and bright, was prepared. When the mouse enters the dark side, we give an electric shock. Then check later, the mouse would go into the dark side or stay in the bright side. In Morris water maze test big pool with platform filled with white-colored tap water. The treated-mouse released into the pool until the mouse find the platform. The escape latency repeated for 5 days. In radial arm maze test, the 8 arms-box were baited with the water in the end of each arm. The water-fasted mouse placed in the center of box and then calculate the number of errors and time for mouse explore the box. and for, Scopolamine-induced test, 4 arms were baited. And we can check the working errors and the escape latency.