산화스트레스 유발 참전복(Haliotis discus hannai)의 박리와 회복에 관한 병리학적 연구

Abstract

Various degree of histological degeneration in the adductor muscle was found in seemingly healthy of cultured abalone, haliotis discus hannai , although the other organs and tissues were quite normal. Any histological evidence for infection of the biological agents was not detected by Giemsa and H & E stains. The frequency of this muscular lesion increased with high temperature. Growth rates increased with the increase of water temperature. Based on these findings, it was hypothesized that muscular degeneration possibly was the result of oxidative stress related with high growth rate. Cultured abalone was artificially applied by infection of carbon tetrachloride for oxidative stress occurrence. From this experiment, similar kind of muscular lesion was obviously induced. Severe swelling of muscle fibers was induced at 12h and 24h postinjection. On the other hand, to investigate the effect of the muscular lesion on abalone’s behavior characteristics, recovery time from application of oxytetracycline used as an exfoliating agent was checked. 3 experimental groups with negative control, positive control and treatment groups were divided. Individuals from all groups were sampled in 0, 12, 24, 72 and 120 hour after the injection. All individuals were treated during 20 second with diluted oxytetracycline just before each sampling times and were measured about recovery time. There were significant difference in recovery time between treatment group and others. With the increase in severity of lesion, the recovery time was lengthened. After 72hrs, recovery time decreased with decrease in severity of swelling of muscle fiber. From these results, it is suggested that the muscular degeneration in seemingly healthy cultured abalone is able to be related to a kind of oxidative stress. These muscular lesions can have a negative effect on the behavioral characteristic and can be related to increase secondary infection or mortality.