잉어에서의 SVCV 검출 및 새로운 주화세포에서의 배양 특성

Abstract

After mass mortality of domestic SVCV was detected from the surviving wild common carp, we analyzed molecular biologically through the detection of SVCV-K1 similar SVCV isolates from wild carp(Cyprinus carpio) of Cheongdo dam reservoir, North Gyeongsang Province. And we checked the this virus belongs to the subgroupⅠa and confirmed the pathogenecity by observed the CPE in vitro culture and cumulative mortality rate in vivo challenged with koi carp. Domestic detection of wild carp will SVCV in the first report, which is considered as a important virus in study of SVCV in domestic cyprinid fish. In addition, in order to higher concentration of SVCV than EPC cell line, israel carp embryo cell and goldfish tail fin cell are established and compared with host cell lines. When inoculated with MEM used for SVCV-K1 on GTF and ICE cell developed in this study, we showed a similar maximum viral copy numbers of SVCV-K1 in the EPC. MEM medium in cell line was changed to serum free medium (SFM). Following inoculation with SVCV-K1 in SFM, CPE was observed in cell lines within 3 days. Certainly, utilization of SFM allowed us to increase susceptibility of GTF, ICE and EPC cell lines to the inoculation of SVCV-K1 in SFM. The susceptibility of the SVCV-K1 in GTF, ICE cells was higher than EPC cell. Although known as the goldfish susceptible to the disease is caused by the SVCV since the disease was recorded in goldfish in a lake in Brazil in 1998, goldfish is lower susceptible to SVCV than carp. This results in higher sensitivity to SVCV-K1 in GTF cell derived from a goldfish than in EPC cell derived from carp is considered a very interesting point. Therefore, inuculation of SVCV-K1 in GTF, ICE cell and using SFM is thought to be higher applied to a variety of research and utilization of SVCV-K1.