천연물 시트러스계의 초임계 이산화탄소에 의한 추출과 아로마테라피의 약리효과

Abstract

Abstract

This thesis describes the effect of aromatherapy on the people under stress and the reduction of stress. Natural citrus used are citron peels cultivated (CCP) in Gohong, Natural citron peel in Gohong (NCP) and poncirus trifoliate peel (PTP) were environmentally friendly extracted at 200 bars and 50oC by supercritical carbon dioxide (SC-CO2) extraction and hexane extraction. The extracts were evaluated in terms of antioxidant activity, antibacterial activity and anti-stress activity. Fatty acid composition of 3 citrus peel oils was found to have the highest percentage of linoleic acid, cis-11,14-eicosadienoic acid, palmitic acid and oleic acid. CCP oil obtained by SC-CO2 extraction was about determined by 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity were 98%. Aromatic oil contents of tea tree, lavender and eucalyptus determined by DPPH free radical scavenging activity were 83, 78 and 70%, respectively. Total phenolic contents (TPC) obtained from CCP oil by SC-CO2 and hexane extraction were 560 (mg/100 g) and 520 (mg/100 g), respectively. Total flavonoid contents (TFC) obtained from SC-CO2 and hexane extraction of CCP oil was 84 (mg/100 g) and 75 (mg/100 g), respectively. Amounts of TPC extracted was larger than that of TFC. Amount of TPC and TFC extracted from the mixture of CCP (MCCP) oil were 300 mg/100 g and 50 mg/100 g, respectively. Mixture of PTP (MPTP), MCCP and mixture of NCP (MNCP) oils showed excellent inhibition activity against bacteria Staphylococcus aureus (+). The Zone of inhibition was in the range of 12 to 16 mm. In addition, MNCP oil showed high inhibition activity against bacteria Pseudomonas aeruginosa (-). The number of volatile compound obtained from CCP oils was 68. The major compounds were terpenes (55.8%), limonene (27.3%), alcohols (15.8%) and linalool (7.3%). MCCP, lavender and eucalyptus blending oil were found to give healing effect of chronic stress, state of tension, depress state, rage and hypochorondria. As a result, antioxidant activity of CCP oil obtained by SC-CO2 extraction was most excellent. In addition, MNCP oil obtained by SC-CO2 extraction showed most good antibacterial activity. MCCP blending oil has a potential to be used for healing of chronic stress, state of tension, depress state, rage and hypochondria. On the other hand, MNCP and tea tree blending oils are expected for use in the area of pimple cosmetics.