청 콜라비의 항당뇨 및 항염증 효과

Abstract

Kohlrabi is a biennial vegetable that belongs to brassicaceae in papaveraceae. Kohlrabi is also called turnip cabbage or corm cabbage. Kohlrabi is an English name which is a compound word of German words Kohl (cabbage) and rabic (turnip). It is a biennial plant differentiated from cabbage and its place of origin is the coastal area of Northern Europe. Its varieties are divided into Asian groups and Western European groups. Asian groups have greyish green leaves and green rough corms. Western European groups that comprise major varieties have green or purple corms with smooth surfaces covered by white waxy substances. Kohlrabi abundantly contains glucosinolates, anthocyanin, and carotenoidin known to have excellent anti-cancer effects and, in particular, its glucosinolates amounts to approximately 25 times of that of radish. Although its has been reported as having antimutagenic actions in in-vitro studies, studies on its physiological activity are insufficient. In this study, the MeOH extract of green Kohlrabi was used to evaluate its ABTS, DPPH, and peroxynitirite eliminating activity and its effect on total phenol measurement. Its antioxidative activity was evaluated through in vitro assays. In addition, its anti-inflammatory activity was investigated by evaluating the generation of NO induced by LPS and MTT toxicity tests using Raw 264.7 cell line obtained from mouse's macrophagocytes. In addition, its anti-diabetic and anti-diabetic complication activity was evaluated through Phosphatase 1B suppressive activity and Lens aldose reductase suppression experiments. In the results, the IC50 values of ABTS, DPPH, peroxynitirite in green Kohlrabi methanol extract were shown to be 787.89±15.19 , 801.03± 0.80, and 132.20± 0.32μg/ml respectively and green Kohlrabi had 4.64 GAE, mg/g extract of phenol content. In the MTT toxicity tests, 89.85%, 91.10%, and 97.26% were shown when MeOH extract concentrations were 200, 400, and 800 (μg/ml) respectively. Therefore, the MeOH extract of green Kohlrabi was judged to have no cytotoxicity. The generation of NO induced by LPS was shown to be 3.8%, 9.7%, and 12.3% at 200, 400, and 800 (μg/ml) concentrations thereby increasing concentration dependently to show weak NO suppressive effects. In Phosphatase 1B suppressive activity experiments, an IC50 value of 207.13 +19.15μg/ml was shown and the control group ursolic acid showed an IC50 value of 5.59±0.44μg/ml. In the Lens aldose reductase suppression experiment, no IC50 value was obtained at concentrations 400, 800, and 1600 (μg/ml) but the suppression increased concentration dependently to 27%, 34%, and 42% respectively thereby showing weak suppressive activity. Through the results of this study, the fact that the MeOH extract of green Kohlrabi has antioxidative and anti-diabetic activity was identified. The fact that it has utility value as a material of functional food can be proposed and its appropriate consumption effect as food can be expected. In addition, this extract can be effective preventive and treating agents as antioxidative and anti-diabetic treatment.