Development of biologically and mechanically induced sponge scaffold composed of marine fish collagen/alginate/chitooligosaccharids for skin tissue regeneration application
- Alternative Title
- 피부조직 재생을 위한 어류콜라겐/알지네이트/키토올리고당 스펀지 세포담체 개발
- Abstract
- An emerging paradigm in wound healing techniques is that a tissue-engineered skin substitute offers an alternative approach to create functional skin tissue. Here we developed a fish collagen/alginate (FCA) sponge scaffold that was functionalized by different molecular weights of chitooligosaccharides (COSs) with the use of 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride as a cross-linking agent. The effects of cross-linking were analyzed by Fourier transform infrared spectroscopy. The results indicate that the homogeneous materials blending and cross-linking intensity were dependent on the molecular weights of COSs. The highly interconnected porous architecture with 160–260 µm pore size and over 90% porosity and COS’s MW driven swelling and retention capacity, tensile property and in vitro biodegradation behavior guaranteed the FCA/COS scaffolds for skin tissue engineering application. Further, improvement of these properties enhanced the cytocompatibility of all the scaffolds, especially the scaffolds containing COSs with MW in the range of 1–3 kDa (FCA/COS1) showed the best cytocompatibility. These physicochemical, mechanical, and biological properties suggest that the FCA/COS1 scaffold is a superior candidate that can be used for skin tissue regeneration.
- Issued Date
- 2015
- Awarded Date
- 2015. 8
- Type
- Dissertation
- Publisher
- Pukyong National University
- Alternative Author(s)
- 파툼 찬디카
- Affiliation
- Department of Biomedical Engineering Center for Marine-integrated Biomedical Technology (BK21 plus) Collage of Engineering Pukyong National University
- Department
- 대학원 의생명융합공학협동과정
- Advisor
- Won-Kyo Jung
- Table Of Contents
- Abstract i
Table of Contents ii
List of Table v
List of Figure vi
1. Introduction 1
1.1. Overview of wounds and the wound healing process 1
1.1.1. Wounds 1
1.1.2. Classical wound healing 2
1.2. Natural materials in the wound healing process 5
1.3. Marine organism-derived materials for wound healing and skin tissue regeneration 6
1.4. Collagen 8
1.5. Alginate 10
1.6. Chitin, Chitosan and chitoologosaccharids 12
2. Materials and methods 15
2.1. Materials 15
2.2. Preparation of skin and isolation of collagen 15
2.3. Amino acid composition 16
2.4. SDS-polyacrylamide gel electrophoresis (SDS-PAGE) 16
2.5. Synthesizing cross-linked FCA/COSs scaffold 17
2.6. Scaffold characterization 19
2.6.1. FTIR analysis 19
2.6.2. Microstructural evaluation (SEM) 19
2.6.3. Porosity of the scaffolds 20
2.6.4. Tensile property analysis 20
2.6.5. Water absorption and retention capacity 21
2.7. In vitro enzymatic degradation 22
2.8. Cell culture 22
2.8.1. Cell viability and proliferation 23
2.8.2. Cell morphology 23
2.8.3. Cell growth behavior and cytocompatibility by florescence microscopy 24
2.8.4. Statistical analysis 24
3. Results 25
3.1. Characterization of fish collagen 25
3.2. Microstructural analysis 28
3.3. Mechanical property 31
3.4. Physicochemical property 33
3.4.1. FTIR 33
3.4.2. Swelling and retention capacity 36
3.4.3. In vitro enzymatic degradation 38
3.5. Biological compatibility 40
3.5.1. Cytotoxicity and proliferation 40
3.5.2. Cell morphology 43
4. Discussion 45
5. Summary 51
References 52
Acknowlagements
- Degree
- Master
- Appears in Collections:
- 대학원 > 의생명융합공학협동과정
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