Effects of dietary ascorbic acid, α-tocopherol, and arachidonic acid on immune responses, hematology, and tissue concentrations in Japanese eel, Anguilla japonica
- Alternative Title
- 뱀장어 친어 사료 내 아스코르브산, 알파 토코페롤 및 아라키돈산 첨가수준에 따른 면역반응, 혈액성상과 조직 내 농도에 미치는 영향
- Abstract
- Four feeding trial were conducted to evaluate the effects of dietary vitamin C (L-ascorbyl-2-monophosphate, AA), E (DL-α-tochpheryl acetate, α-Toc) and arachidonic acid (ARA, 20:4n-6) levels on growth performance, whole-body and liver proximate compositions, tissue concentrations, hematological parameters and non-specific immune responses in Japanese eel, Anguilla japonica. In experiment 1, the optimum dietary AA could be greater than 410.3 but less than or equal to 1005 (mg AA kg-1) based on broken-line regression analysis of liver and muscle AA concentration, in male broodstock Japanese eel. In experiment 2, the optimum dietary α-Toc could be greater than 199.7 but less than or equal to 212.9 (mg α-Toc kg-1) based on broken-line regression analysis of testes and liver α-Toc concentration, in male broodstock Japanese eel. In experiment 3, the optimum dietary ARA level with the 1:2 ratio of EPA:DHA could be greater than 0.43% diet but less than or equal to 0.71% based on the broken-line regression analysis and the ANOVA test, respectively. In experiment 4, the optimum dietary ARA level with the 1:1 ratio of EPA:DHA could be greater than 0.48% diet but less than or equal to 0.73% based on the broken-line regression analysis and the ANOVA test of weight gain, respectively.
- Issued Date
- 2015
- Awarded Date
- 2015. 8
- Type
- Dissertation
- Publisher
- 부경대학교 대학원
- Affiliation
- 부경대학교
- Department
- 대학원 수산생물학과
- Advisor
- 배승철
- Table Of Contents
- Table of contents
Abstract iii
Dedication ix
Acknowledgment x
List of Tables xii
List of Figures xv
CHAPTER 1. Literature Review 1
1. Nutrient requirements in Japanese eel 5
2. Nutritional factors affecting reproduction and immune responses in broodstock fish 7
3. References 8
CHAPTER 2. Effects of dietary vitamin C levels on tissue ascorbic acid concentration,
hematology, non-specific immune responses and gonad histology in
broodstock male Japanese eel, Anguilla japonica………………………………11
1. Introduction 12
2. Materials and methods 14
3. Results and Discussion 18
4. References 22
5. Tables and Figures 29
CHAPTER 3. Effects of dietary vitamin E levels on tissue α-tocopherol concentration, hematology, non-specific immune responses and gonad histology in broodstock male Japanese eel, Anguilla japonica 38
1. Introduction 39
2. Materials and methods 41
3. Results and Discussion 45
4. References 49
5. Tables and Figures 55
CHAPTER 4. Effects of dietary arachidonic acid levels with the 1:2 ratio of
EPA:DHA in female subadult Japanese eel, Anguilla japonica…...…...64
1. Introduction 65
2. Materials and methods 67
3. Results and Discussion 71
4. References 75
5. Tables and Figures 82
CHAPTER 5. Effects of dietary arachidonic acid levels with the 1:1 ratio of EPA:DHA in female subadult Japanese eel, Anguilla japonica…………91
1. Introduction 92
2. Materials and methods 94
3. Results and Discussion 98
4. References 102
5. Tables and Figures 109
CHAPTER 6. Conclusions and future perspectives 118
CHAPTER 7. Appendix 121
- Degree
- Doctor
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