PUKYONG

GUIDE OAK 국가리포지터리 부경대학교

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Abstract: A total of 34 microalgae species were isolated from the digestive tract of hard clam (Meretrix lusoria). Among them, 32 species were identified by microscopic observation and comparison with reference pictures. Two unidentified microalgae names U4 and U5 were further characterized. The 18S rRNA genes were successfully amplified by common 18S rRNA gene primers from these isolates. Sequence analysis of these amplified DNA form the solate U4 and U5 showed 97% and 98% sequence similarity to those of Neorhodella cyanea and Minutocellus polymorphus, respectively. Scanning electron microscopic observation also confirmed the species identification. Both Neorhodella sp. and Minutocellus sp. showed the highest growth at 18 oC with the specific growth rate and number of divisions per day of 1.62±0.39 and 2.24, and 0.39±0.01 and 0.56, respectively.

URI: https://repository.pknu.ac.kr:8443/handle/2021.oak/12853
http://pknu.dcollection.net/jsp/common/DcLoOrgPer.jsp?sItemId=000002227902

Table Of Contents: Tables of contents i
List of Figures iii
List of Tables iv
Abstract v
Introduction 1
Materials and Methods 5
Classification and isolation of microalgae 5
Molecular identification of unknown microalgae 6
Scanning electron microscopy morphology analysis 7
Growth rate measurement 8
Results 9
Classification and isolation of microalgae 9
Molecular identification of unknown microalgae 12
Scanning electron microscopy morphology analysis 16
Growth rate measurement 18
Discussion 22
Conclusion 26
References 27
Appendix 34
Acknowledgement 40

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