Preparation and Characterization of Carbon Quantum Dot-Loaded Biocompatible Polymer Particles
- Alternative Title
- 탄소양자점 함유 생체적합성 고분자 나노입자의 제조
- Abstract
- Carbon quantum dots (CQDs) are carbon based materials exhibiting marked photophysical properties depending on the size. However, solvent compatibility and chemostability are still issues to improve. We introduced hydrophobic units to CQDs so that functionalized CQDs can be incorporated within polymeric micelles in aqueous media. Successful incorporation of CQDs into micelles and their photophysical properties were characterized by UV/Vis and fluorescence spectroscopy.
- Issued Date
- 2016
- Awarded Date
- 2016. 2
- Type
- Dissertation
- Publisher
- The Graduate School, Pukyong National University
- Affiliation
- The Graduate School, Pukyong National University
- Department
- 대학원 화학과
- Advisor
- Minseok Kwak
- Table Of Contents
- Contents i
Abstract iii
List of Figures v
List of Tables viii
Chapter 1: Introduction 1
1.1 Background and motivation 1
1.2 Objective and research method 5
1.3 Outline and contribution of thesis 7
Chapter 2: CQD Incorporation into F127 sIPN Micelle 8
2.1 Literature review 8
2.1.1 Carbon quantum dots (CQDs) for live cell imaging 8
2.1.2 Synthesis of hydrophobic CQDs 11
2.1.3 Pluronic block copolymers as encapsulation agent for hydrophobic material 13
2.2 Methodology 16
2.2.1 Materials 16
2.2.2 Instruments 16
2.2.3 Experimental 17
2.2.3.1 Hydrophobic moieties functionalization 17
2.2.3.2 IR spectroscopy 18
2.2.3.3 F127 encapsulation and sIPN formation 18
2.2.3.4 Incorporation of hydrophobic CQDs within
F127 sIPN micelle 22
2.2.3.5 UV/Vis and fluorescence spectroscopy 24
2.3 Result and discussion 24
2.3.1 Synthesis of hydrophobic CQDs 24
2.3.2 Incorporation of hydrophobic CQDs within F127
micelle 30
2.3.3 Stabilization of F127 micelle through sIPN formation 41
Chapter 3: SERS of DNA Functionalized Reporter 46
3.1 Literature review 46
3.1.1 Raman scattering and surface enhanced Raman
scattering 46
3.1.2 Hydrophobicity of nano materials 50
3.1.3 DNA as self-assembling molecule probe 51
3.2 Methodology 51
3.2.1 Materials 51
3.2.2 Instruments 52
3.2.3 Experimental 52
3.2.3.1 Quasi-3D nanoholes gold array 52
3.2.3.2 Thiolated DNA functionalization as Raman
reporter 53
3.2.3.3 Raman spectroscopy and data processing 54
3.3 Result and discussion 55
3.3.1 SERS selective substrate 55
3.3.2 Effect of solvent variation 56
3.3.3 Effect of incubation time variation 58
3.3.4 Sequence specificity 59
3.3.5 Enhancement factor (Ef) calculation 60
Chapter 4: Conclusions and Outlooks 64
4.1 Conclusions 64
4.2 Outlooks 65
References 66
Acknowledgment 76
- Degree
- Master
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