Semi-interpenetrating Network Formation of F127: Versatile Loading and Optimized Stabilization
- Alternative Title
- 반상호침투 가교구조 F127 나노입자 제조 최적화와 형광체 담지
- Abstract
- We present a method to efficiently stabilize F127 micelles via semi-interpenetrating network (sIPN) formation. First, pentaerythritol tetraacrylate loaded in the hydrophobic core of micelles was crosslinked by UV-irradiation to form sIPN resulting in stabilized soft nanoparticles against temperature and concentration changes. Stability and size of the particles were evaluated by spectroscopy techniques. Various chemical compositions and experimental conditions were also tested to optimize sIPN formation of the polymer. The optimized experimental conditions and chemical composition for sIPN formation of polymer can be summarized as follows: 6 minutes is found as a minimum irradiation time, 2.5% of PETA is enough to form good nano-network, the sIPN formation need to be conducted at the elevated temperature (50 °C) to help PETA internalization, pyrene content doesn’t have significant effect into stability of micelle and 10 mL and 1 mL show good sIPN formation for sample prepared at the 20 mL and 5 mL vial respectively.
In order to test the versatile properties of sIPN nanoparticles to load various kinds of hydrophobic compound, eleven organic fluorescence probes were also loaded into sIPN nanoparticles via three different methods. The resulting nanoparticles were capable of loading organic fluorescence probe which are represented as absorption and fluorescence spectra and fluorescence quantum yield value. sIPN nanoparticle is also known to has a good biocompatibility as shown in the in vitro and in vivo imaging .
In addition, the versatile loading properties of sIPN were tested to disperse and stabilize inorganic material, quantum rods (QR), in the aqueous environment. The QR-sIPN was formed via film hydration method and it was characterized by spectroscopic method, TEM imaging and cellular internalization into TC1 cells. The stability test (CMT test) of QR-sIPN show that QR-sIPN can keep their micellar structure even after stored at the low temperature for long period (54 days). The TEM image reveals that the size and shape of QR-sIPN is just slightly different from pristine QR in the organic solvent. The cellular internalization of QR-sIPN again show good biocompatibility of sIPN nanoparticles.
- Issued Date
- 2016
- Awarded Date
- 2016. 2
- Type
- Dissertation
- Publisher
- The Graduate School, Pukyong National University
- Affiliation
- The Graduate School, Pukyong National University
- Department
- 대학원 화학과
- Advisor
- Minseok Kwak
- Table Of Contents
- Dedications
Contents
Abstract
Abstract (Korean)
List of Figures
List of Tables
Chapter 1: Introduction 1
1.1 Research overview 1
1.2 Literature review 3
1.2.1 Polymeric micelle as drug delivery material 3
1.2.2 Semi-interpenetrating polymer network (sIPN) 6
1.2.3 Hydrophobic environment tracking by pyrene solubilization method 10
1.2.4 Cellular internalization of polymeric micelle material 11
1.2.5 Water-dispersible QR via Pluronic micelle encapsulation 11
1.3 Outline of thesis 13
Chapter 2: sIPN Formation in the core of F127 Micelle: Optimization and stability studies 16
2.1 Methodology 16
2.1.1 Materials 16
2.1.2 Instruments 16
2.1.3 Experimental 17
2.1.3.1 Solution preparation 17
2.1.3.2 Formation of sIPN in the core of F127 micelle 18
2.1.3.3 CMT test 18
2.3.1.4 Optimization of sIPN formation 19
2.3.1.5 Stability test of F127 sIPN 21 21
2.2 Discussion 22
2.2.1 Pyrene absorption and florescence intensity in the F127 sIPN 22
2.2.2 CMT test to confirm sIPN formation 23
2.2.3 Effect of PETA content 25
2.2.4 Effect of irradiation time 27
2.2.5 Effect of reaction temperature 27
2.2.6 Effect of pyrene content 31
2.2.7 Effect of sample volume 33
2.2.8 Stability test of F127 sIPN 37
2.3 Conclusion 41
Chapter 3: Organic fluorescence probe loaded sIPNs 42
3.1 Methodology 42
3.1.1 Materials 42
3.1.2 Instruments 43
3.1.3 Experimental 43
3.1.3.1 Preparation of organic fluorescence probe-loaded sIPNs 45
3.1.3.2 Absorption and fluorescence spectroscopy of organic fluorescence probe loaded sIPNs 48
3.1.3.3 Fluorescence quantum yield measurement 48
3.1.3.4 Cell uptake experiment 48
3.1.3.5 Animal imaging 49
3.2 Discussion 49
3.2.1 Photophysical properties of organic fluorescence probe loaded sIPNs 49
3.2.2 Fluorescence quantum yield value (Φf) of organic fluorescence probe loaded sIPNs 54
3.2.3 Cellular internalization of organic fluorescence probe loaded sIPNs 57
3.2.4 Animal imaging (in vivo and ex vivo) 59
3.3 Conclusion 61
Chapter 4: Formation of water dispersible QR-F127 sIPN 62
4.1 Methodology 62
4.1.1 Materials 62
4.1.2 Instruments 62
4.1.3 Experimental 63
4.1.3.1 Formation of QR-Pluronic dispersions 63
4.1.3.2 Formation of QR-F127 sIPN: Optimization of chemical composition 63
4.1.3.3 Stability test of QR-F127 sIPN nanoparticles 64
4.1.3.4 Spectroscopic analysis 64
4.1.3.5 TEM imaging 65
4.1.3.6 In vitro TC1 cell experiment 65
4.2 Discussion 66
4.2.1 Formation QR-F127 micelles 66
4.2.2 QR-F127 sIPN formation 68
4.2.3 Stability test of QR-F127 sIPN 70
4.2.4 TEM imaging of QR-F127 sIPN 72
4.2.5 In vitro TC1 cells experiment of QR-F127 sIPN 73
4.3 Conclusion 75
Chapter 5: General Conclusions and Outlooks 77
Chapter 6: References 80
Acknowledgment 87
- Degree
- Master
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