Synthesis and antibacterial application of polymer encapsulated layered double hydroxides composites via RAFT polymerization

Abstract

A facile route has been developed to prepare Gentamicin conjugated poly (oligo (ethylene glycol) methacrylate)-grafted-layered double hydroxides (LDHs-g-POEGMA-GE) by the reversible addition fragmentation chain transfer (RAFT) polymerization for the antibacterial claim. Initially, the layered double hydroxides (LDHs) surface was modified by S′-(3-trimethoxysilyl) propyl trithiocarbonate (BTPT). Then, poly (oligo (ethylene glycol)methacrylate) (POEGMA) was grafted onto the LDHs by RAFT polymerization using oligo (ethylene glycol methacrylate) as a monomer. The hydroxyl end groups of the grafted POEGMA brushes were converted into carboxyl groups, and Gentamicin (GE) was anchored on the LDHs by the carbodiimide reaction. The LDHs-g-POEGMA composites were characterized by the FT-IR, SEM, GPC, TGA, XRD analysis. The in-vitro assay with Staphylococcus aureus bacteria presented the high antibacterial activity of the LDHs-g-POEGMA-GE composites. The LDHs-g-POEGMA-GE composites have shown potential applications in the biomaterial field.

항균적인 작용을 위한 Gentamycin과 poly (oligo(ethylene glycol) methacrylate)-grafted-layered double hydroxides (LDHs-g-POEGMA-GE)를 reversible addition fragmentation chain transfer polymerization (RAFT) 중합법을 이용하여 합성하였다. 초기에, LDHs 표면은 S’-(3-trimethoxysilyl) propyl trithiocarbonate 에 의하여 개질되었다. 그리고, POEGMA는 단량체로 사용된 oligo (ethylene glycol methacrylate)를 RAFT중합법에 의해서 LDHs 위에 graft 하였더니, graft된 POEGMA 가지의 hydroxyl 말단그룹은 carboxyl 그룹으로 변환되었고, Gentamicin은 esterification 반응에 의하여 LDHs 위에 고정되었다. LDHs-g-POEGMA 복합체들의 특성은 FT-IR, SEM, GPC, TGA, XRD 분석을 통해 확인하였다. In-vitro assay로 사용된 황색 포도상 구균 박테리아는 LDHs-g-POEGMA-GE 복합체들의 높은 항균적인 활동으로 나타났다. 이러한 LDHs-g-POEGMA-GE 복합체들은 생체재료 분야에서 잠재적인 응용 가능성을 보여 주었다.