The histological effects of bio-energy water on degenerated adductor muscle found endemically in cultured abalone, Haliotis discus hannai

Abstract

This study was conducted to investigate the histological effect of bio-energy water on degenerated adductor muscle found endemically in cultured abalone (Haliotis discus hannai). Adult abalones averaging of total weight 60.15 ± 17.55 g, shell weight 16.93 ± 4.09 g, shell length 8.12 ± 0.76 cm, shell width 5.49 ± 0.46 cm, and shell height is 1.73 ± 0.76 cm was obtained from a farm in South Jeolla Province Wando County. H. discus hannai were divided into two treatment groups: first group was applied with the bio-energy water whilst the second group was applied with the general sea water. Abalones were collected in 5 samplings, 10 heads per sampling. Abalones were dissected to examine adductor muscle and measured. Adductor muscle tissues were fixed, re-fixed, processed, embedded with paraffin, sectioned and stained with H & E (Hematoxylin & Eosin) for light microscopy. Adductor muscle in 1st sample was found to consider as the whole necrotic muscle atrophy all of 10 individuals. Second sample as a control group in 1st week of bio-energy water, adductor muscle of abalones were found to be swelling, vitrified (hyalinization) and vacuolization. These findings showed atrophy and loss of muscle significantly in the both the first and second group. In the 2nd week, after bio-energy water was applied to the third sampling, abalones were not shown different with second sampling in general water group. However, abalone in bio-energy water showed that the muscle atrophy and losing had significantly improved with general water group. There was also noticeable increasing in the density of the muscle thickness and the muscle fibers vacuolization had disappeared of 9 individuals being investigated in these findings. Bio-energy water indicated the lesions, which confirmed, namely, shell mounting portion of the muscle adductor muscle edema, vitrification (hyalinization), vacuolization, atrophy and loss findings had improved significantly in the 3rd week and there was a significant improvement compared to the 2nd week. As the Adductor muscle grew thicker the density of the muscle in all cases and the vacuolization findings of muscle fibers almost disappeared. The 5th and final sampling after 8 weeks, showed the level in the adductor muscle and the muscle fibers in all cases had a complete clear recovery phase. The underlying mechanism for the striking effect for the muscle recovery could be possibly derived from the unique power of water molecules which makes all the bio-molecules sustain original normal structures of them for the proper functions in the tissue. This natural power of water molecule has long been hidden so far and should be deeply considered to be the main body of immune. From these results, the bio-energy water used in this study was highly recommendable for the recovery of necrotic muscle lesion of cultured abalone.