젖소의 분변으로부터 분리한 유산균의 tyrosinase 억제 및 항산화 효과

Abstract

Overproduction and accumulation of melanin pigments cause a number of skin diseases. Reactive oxygen species (ROS), generated by metabolic intermediates in melanin biosynthesis, cause various diseases including cancer and heart diseases. Therefore, both tyrosinase inhibitors and anti-oxidants are important for the treatments of diseases, occurred by melanins accumulation and ROS. This study was aimed at the tyrosinase inhibitory and antioxidative effects of four different lactic acid bacteria (LAB) strains, isolated from dairy cow feces. We have isolated four different LAB strains designated as Enterococcus sp. EA3, Enterococcus sp. EB2, Pediococcus sp. PC2, and Pediococcus sp. PD3. To investigate optimal culture conditions of isolated LAB strains, we have performed the measurements of tyrosinase inhibitory and antioxidative activities against growth times (hour), temperatures (℃), pH, and NaCl concentrations (％) using culture filtrates of LAB. The tyrosinase inhibitory activities of LAB showed that Enterococcus sp. EA3 was about 65% at its optimum culture condition (14 hour, 30℃, pH 8 and 0% NaCl), Enterococcus sp. EB2 about 65% (12 hour, 30℃, pH 9 and 0% NaCl), Pediococcus sp. PC2 about 80% (20 hour, 30℃, pH 6 and 0% NaCl), and Pediococcus sp. PD3 about 80% (20 hour, 30℃, pH 8 and 0% NaCl), respectively. In addition, anti-oxidative activities of four different LAB strains showed approximately more than 30% at optimal conditions. From the results, the culture filtrates obtained from four LAB strains can be a potential agent for developing antioxidants and inhibitors for tyrosinase. Furthermore, this study will be applied for developing skin care cosmetics.