Amphidinium carterae의 대량배양을 통한 항곰팡이성 물질 추출

Abstract

Marine dinoflagellates produce diverse bioactive compounds in which amphidinols, antifungal and hemolytic compounds are known as natural products from the genus Amphidinium. This study was conducted to separate and purify the amphidinols (natural anti-fungal substrates) in marine microalgae Amphidinium carterae.

A. carterae strain was grown unialgally in f/2 medium and the culture processes were separated by the preservation (stock culture), the middle culture (15 L) and the mass culture (over 300 L) systems of Fisheries Science & Technology Center, Pukyong National University. When the cell density in mass culture reached to peak at 100 × 104 cells/mL, it was harvested by the auto-filtration (using Synopex membrane filter)system.

The concentrated cells were extracted and separated into the first antifungal substance with MeOH, CHCl3, and BuOH treatment methods, separately. And then it was separated by 60 %, 85 %, 100 % MeOH using the ODS-Q3 column, and purified it by using a Sephadex LH-20 column. Finally, the fractions having antifungal activities were chromatographed on a RPAQUEOUS column by the HPLC system.

During the above purification procedures, antifungal activities were tested by a paper disk method against Aspergillus niger. In these processes, antifungal activities appeared at first in the elute of 85% MeOH extract on the ODS-Q3 column and showed clear responses on the elutes of large peaks of LH-20 column.

These results mean that amphidinols showing antifungal activities are produced from A. carterae strain of Korea and the more detailed researches should be anticipated on the structural analyses and applications to the biological and medical sciences.