Anti-inflammatory Effect of an Ethanolic Extract of Myagropsis yendoi in Lipopolysaccharide-Stimulated BV-2 Microglia Cells
- Abstract
- Myagropsis yendoi is a brown alga that was shown to have anti-inflammatory. In this study, the molecular mechanism of the anti-inflammatory action of Myagropsis yendoi ethanolic extracts (MYE) was investigated using lipopolysaccharide (LPS)-stimulated microglia BV-2 cells. MYE inhibited LPS-induced nitric oxide (NO) production in a dose-dependent manner and suppressed the expression of inducible nitric oxide synthase (iNOS) in BV-2 cells. MYE also reduced the production of pro-inflammatory cytokines in LPS-stimulated BV-2 cells. LPS-induced nuclear factor-B (NF-B) transcriptional activity and NF-B translocation into the nucleus were significantly inhibited by MYE treatment through the prevention of the degradation of inhibitor B-α (IB-α). Moreover, MYE inhibited the phosphorylation of Akt, ERK, JNK and p38 MAPK in LPS-stimulated BV-2 cells. The results indicate that the MYE can be considered as a potential source of therapeutic agents for neuro-inflammatory diseases.
- Issued Date
- 2014
- Awarded Date
- 2014. 2
- Type
- Dissertation
- Publisher
- 부경대학교
- Affiliation
- 글로벌수산대학원
- Department
- 글로벌수산대학원 국제수산과학협동과정
- Advisor
- 김형락
- Table Of Contents
- Tables of i
List of Figures iii
List of Tables iv
Abstract v
1. Introduction 1
2. Materials and Methods 5
2.1. Plant material and preparation of MYE 5
2.2. Reagents 5
2.3. Cell cultures and sample treatment 6
2.4. Cytotoxicity assay 6
2.5. Measurement of nitric oxide and pro-inflammatory cytokines 7
2.6. Western blot analysis 8
2.7. Preparation of cytosolic and nuclear extracts 9
2.8. Reverse transcription-polymerase chain reaction (RT-PCR) 10
2.9. Immunocytochemical analysis 12
2.10. Statistical Analysis 13
3. Results 14
3.1. Effect of MYE on NO production in LPS- stimulated BV-2 cells 14
3.2. Effect of MYE on the expression of iNOS in BV-2 cells 17
3.3. Effect of MYE on the production of TNF-α and IL-6 in LPS-treated BV-2 cells 20
3.4. Effect of MYE on the nuclear translocation of NF-B in LPS-stimulated BV-2 cells 23
3.5. Effect of MYE on the activation of JNK, ERK, AKT and p38 in LPS-stimulated BV-2 cells 27
4. Discussion 29
5. Conclusions 34
6. Acknowledgement 35
7. References 37
- Degree
- Master
- Appears in Collections:
- 글로벌수산대학원 > 국제수산과학협동과정
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