해조류 및 목질계 바이오매스 가수분해물을 이용한 Chlorella saccharophila 배양 및 유용물질 생산

Abstract

This study was culture of Chlorella saccharophila and production of useful materials using lignocellulosic and marine biomass hydrolysates. First studied optimization of C. saccharophila culture medium. This study factor was medium type, culture type, inoculum size, sugar kind and nitrogen source type. Culture was conducted in a shaking incubator and the reaction temperature is 25℃ and agitated at 120 rpm. The used carbon sources tested in this study were fructose, mannose, lactose, sucrose, xylose, galactose, glycerol and some concentration glucose. Nitrogen sources were NH4NO3, urea, (NH4)2SO4, casein, yeast extract, peptone, corn steep solid and some concentration KNO3 and NaNO3. The 30 g/L glucose and 0.950 g/L NaNO3 were found to be the best carbon and nitrogen source and concentration. Experiment result, oil content and chlorophyll content was 57.00% and 4.73% on 12 day. Used lignocellulosic hydrolysates, not dried rapeseed straw oil content and chlorophyll content was 31.75% and 5.56%, and after activated carbon treatment rapeseed straw oil content was 31.15% and barley straw chlorophyll content was 5.24% on 12 day. And used marine hydrolysates, after activated carbon treatment U. pinnatifida oil and chlorophyll content was 31.50% and 6.26% on 12 day.