Adipogenesis inhibitory effect of Skipjack Tuna extract in 3T3-L1 adipocytes

Abstract

In recent years, obesity can cause heart disease, type 2 diabetes, obstructive sleep apnea, certain types of cancer and osteoarthritis, and become a global health problem. Therefore researchers want to find functional drugs to improve anti-obesity. In this study, it was focused on adipogenesis inhibitory effect of tuna peptides from Skipjack tuna extract in 3T3- L1 adipocytes. First, MTS assay indicated that tuna peptides toxicity to 3T3-L1 cells was not detected. Subsequently, this experiment was also measured glucose uptake, triglyceride levels and lipid droplets using Oil Red O staining. As a dose-dependently attenuated result, that confirmed the Skipjack tuna extract inhibits adipocytes differentiation. It is confirmed the expression of obesity-associated genes by western blot analysis as well. Skipjack tuna extract significantly reduced the expression levels of CCAAT/enhancer-binding protein-α (C/EBPα), CCAAT/enhancer-binding protein-δ (C/EBPδ), peroxisome proliferator-activated receptor-γ (PPARγ), fatty acid synthase (FAS), lipoprotein lipase (LPL), leptin and cluster of differentiation 36 (CD36) adipocytes marker genes. On another hand TP could suppress adipocytes differentiation by inducing Wnt-10b overexpression through Wnt canonical signal pathway. Consequently, the data suggest that Skipjack tuna extract decrease lipid components and adipogenesis and reduce differentiation of 3T3-L1 adipocytes, and these characteristics may be an effective function drug to treat obesity-related metabolic diseases.