Anti-obesity effect of boiled tuna extract on C57BL/6N mice fed a high fat diet

Abstract

Obesity is one of common issues in the world, that has been increasing significantly recently. Recent in vitro studies have demonstrated that tuna which contained of bioactive compound can decreased as significantly the expression level of C/EBP α and PPAR γ as a marker of adipogenesis genes. This study confirms about the effectiveness of tuna as a solution to prevent obesity, which could be offered to people for food consumption. Through in vivo studies, we proved using C57BL/6N mice under high fat diet by feeding them tuna in various concentrations (50 mg/kg, 100 mg/kg, 200 mg/kg, and 400 mg/kg) for ten weeks. The sample of blood and liver tissue have been analysed by using several KITs and western blot analysis. The results show that feeding tuna treatments can constantly enhance HDL-C, inversely reduced weight gain, leptin, which give damages for lowering appetite, decreased glucose, triglycerides, TC, LDL-C, insulin, and some marker in the blood like ALT and AST. It is followed in the liver as a hallmark of heart disease that has a link with obesity with reduced GOT and GPT and elevates SOD and CAT. Simultaneously, the genes expression results also supported that which gene related to obesity which down-regulated adipogenesis related genes and up-regulated the genes related to obesity like AMPK are expressed. Based on these results, it showed that tuna extract could be potential novel treatment as anti-obesity treatment that effective to reduce obesity.