PUKYONG

극지 미세조류의 세포외 다당 분비 및 특성

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Abstract
Arctic sea ice algae are known to produce essential proteins to prevent freezing in the ice to survive. However, research on sea ice algae extracellular polysaccharides (EPS) is lagging behind. Microalgae have been reported to be about 30,000 species currently exist in the world. The polar region is an area where the environment change can happen rapidly. As a result, structural change of sea ice will affect the ecosystem, thereby causing a large ripple effect. Therefore, reduction of the ice according to the global warming has become a very big issue. However, research on EPS, which is secreted by sea ice algae is currently very limited. So I analyzed the production of EPS due to changes in the environment (temperacture and salinity) and the characteristic of secreted EPS. As a result, microalgae EPS is abundant of fucose, rhamnose, xylose and the degree of sulfation is 40%, so that is sulfuric acid fucan. Analyzing spectroscopic characteristics of the EPS using a FT- IR, NMR showed similar results to the chemical analysis of the EPS. Further sea ice micro algae EPS has few or no ice recrystallization inhibition activity. Also this study showed that the EPS’s antibacterial activity is very high. EPS produced by algae and bacteria are considered different. Therefore, If the experiment is proceeding more, it clarifies the detailed structure of each of the EPS and perform research on various physiological activities, that provide an important basis for identifying the role of EPS in sea ice.
Author(s)
황태경
Issued Date
2016
Awarded Date
2016. 8
Type
Dissertation
Keyword
extracellular polysaccharides Arctic sea sea ice algae
Publisher
부경대학교 대학원
URI
https://repository.pknu.ac.kr:8443/handle/2021.oak/13300
http://pknu.dcollection.net/jsp/common/DcLoOrgPer.jsp?sItemId=000002299512
Affiliation
부경대학교 대학원
Department
대학원 화학과
Advisor
김학준
Table Of Contents
1. 서론1
1. 1 극지의 환경 1
2. 연구 목적7
3. 재료 및 방법
3.1 균주 및 배지조성8
3.2 배양조건9
3.3 EPS 추출 및 측정10
3.4 생존률 측정11
3.5 Phyto-PAM을 이용한 광합성 활성 측정12
3.6 당 및 단백질 함량 측정12
3.7 EPS의 성분 원소 분석 및 크기배제 크로마토그래피13
3.8 EPS 단당성분 및 글리코시딕 결합분석13
3.9 EPS 황산 및 유론산 분석14
3.10 EPS FT-IR 측정15
3.11 EPS의 NMR 측정15
3.12 EPS 항산화 활성 측정16
3.13 EPS의 얼음 결합 특성 분석18
3.14 EPS의 동결보존 활성 측정22
4. 결과 및 고찰
4.1 극지 해빙 미세조류의 배양 조건에 따른 EPS 생산 및 대량 배양 시스템 구축23
4.2 극지 해빙 미세조류의 EPS 추출 및 특성 분석35
4.3 EPS 얼음결합 및 생리활성 특성 분석45
5. 요약58
6. 참고문헌60
Degree
Master
Appears in Collections:
대학원 > 공업화학과
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