Effects of Salinity and Copepods on Artificial Seed Production of the Rockworm Marphysa sanguinea (Polychaeta: Eunicidae)
- Alternative Title
- 바위털갯지렁이(Marphysa sanguinea: Eunicidae) 인공종묘생산에 대한 염분과 요각류의 영향
- Abstract
- The rockworm, Marphysa sanguinea (Montagu, 1815) (Polychaeta: Eunicidae), one of high economic value species, has been increasing in demand for fishing bait as well as a living food resource for fin fish and shrimp. Rockworm farms have recently started developing in order to understand and promote to complete the artificial seed production techniques. In this study, three experiments were conducted to investigate the effects of salinity and copepods on artificial seed production of M. sanguinea in Fisheries Science and Technology Center (FSTC), Pukyong National University.
The first experiment was effects of temperature, water level, tidal time, sunshine and humidity on larval release of artificial seed production, conducted during 2015 and 2016. The results showed that the total of larval release in 2015 showed 2.02 fold and 2.72 fold higher than in 2016 of artificial seed production on temperature regulated and natural seawater, respectively. The larval release depended on the tides, particularly semilunar rhythm and seawater level. The second experiment was conducted to investigate the effects of salinity on growth, survival rates and biochemical compositions of M. sanguinea. The suitable salinity tolerance was 25 to 30 psu for different stages from larvae to adult in the semi-recirculation system, particularly, 25 psu for juveniles and adults, 30 psu for larvae. Moreover, the biochemical compositions of the rockworm at different salinities (15 to 35 psu) were also investigated. Saturated, monounsaturated and polyunsaturated acids were detected in all tested worms from different salinity experiments. There was no significant difference in fatty acids among the treatments and 27 fatty acids were recorded at all tested salinity levels and abundance of amino acid components was detected. The third experiment was removal of predators, particularly copepods to induce the growth and survival rates of M. sanguinea by physiological methods, including exposure interval by tidal water drainage and trichlorfon also was conducted. The results showed that exposure interval by tidal water drainage (once in 3h for couple of days) and trichlorfon (1 to 3 ppm) are the effective methods to remove copepods out of the rearing systems.
The results of this study suggested that effects of salinity and copepod removal could be vitally important in contributing to the successful artificial seed production of M. sanguinea. This study also proved that M. sanguinea could be a potential living food candidate for aquaculture.
- Issued Date
- 2017
- Awarded Date
- 2017. 2
- Type
- Dissertation
- Publisher
- 부경대학교 대학원
- Affiliation
- 부경대학교 대학원
- Department
- 대학원 수산생물학과
- Advisor
- 김창훈
- Table Of Contents
- Chapter 1. General Introduction 1
Chapter 2. Larval Production of Marphysa sanguinea under Laboratory Conditions 8
2.1. Introduction 8
2.2. Materials and methods 11
2.2.1. Larval production in temperature regulated seawater 11
2.2.2. Larval production in natural seawater 12
2.3. Results 15
2.3.1. Larval production in temperature regulated seawater 15
2.3.1.1. Total larval production in temperature regulated seawater 15
2.3.1.2. Relationship between larval production and environmental factors of artificial seed production by temperature regulated seawater 15
2.3.2. Larval production in natural seawater 22
2.3.2.1. Total larval production in natural seawater 22
2.3.2.2. Relationship between larval production and environmental factors in artificial seed production by natural seawater 22
2.4. Discussion 29
2.4.1. Larval characteristics 29
2.4.2. Relationship between larval release and tidal phenomenon 29
2.4.3. Relationship between larval release and semilunar rhythm 30
Chapter 3. Effects of Salinity on Survival, Growth Rates and Biochemical Compositions of Marphysa sanguinea 32
1.1. Introduction 32
1.2. Materials and Methods 35
1.2.1. Experiment 1: Effects of salinity on survival and growth rates of M. sanguinea larval stage 35
3.2.1.1. Larval isolation 35
3.2.1.2. Sediment preparation 35
3.2.1.3. Biofloc water preparation 35
3.2.1.4. Experimental preparation for salinity 36
3.2.1.5. Feeding condition 37
3.2.2. Experiment 2: Effects of salinity on survival and growth rates of M. sanguinea juvenile stage 42
3.2.2.1. Worm selection 42
3.2.2.2. Experimental facility 42
3.2.2.3. Experimental design 43
3.2.3. Experiment 3: Effects of salinity on survival and growth rates of M. sanguinea adult stage 49
3.2.3.1. Adult worms selection 49
3.2.3.2. Experimental design for adult stage 49
3.2.4. Experiment 4: The biochemical compositions of M. sanguin adult body at different salinities 50
3.2.4.1. Biochemical analysis 50
3.2.4.2. Fatty acids 51
3.2.4.3. Amino acids 52
3.2.5. Water analysis methods 52
3.2.6. Data analysis 53
3.3. Results 58
3.3.1. Experiment 1: Effects of salinity on survival and growth rates of rockworm M. sanguinea larval stage 58
3.3.1.1. Survival rate of M. sanguinea larvae in semi-recirculation system from 0 - 60 days 58
3.3.1.2. Growth performance of M. sanguinea larvae in semi-recirculation system from 0 - 60 days 58
3.3.1.3. Water quality parameters in larval rearing bottles 58
3.3.1.4. Biofloc system water quality parameters 59
3.3.2. Experiment 2: Effects of salinity on survival and growth rates of M. sanguinea juvenile stage 64
3.3.2.1. Survival rate of M. sanguinea juveniles for 3 months 64
3.3.2.2. Growth rate of M. sanguinea juveniles for 3 months 64
3.3.2.3. Specific growth rate of M. sanguinea juveniles for 3 months 64
3.3.2.4. Water quality parameters for juveniles 65
3.3.3. Experiment 3: Effects of salinity on survival and growth rates of M. sanguinea adult stage 70
3.3.3.1. Survival and growth rates of M. sanguinea adults 70
3.3.3.2. Water quality parameters for M. sanguinea adults 70
3.3.4. Experiment 4: Biochemical composition of M. sanguinea adults at different salinities 74
3.3.4.1. Biochemical composition of M. sanguinea adults 74
3.3.4.2. Fatty acid profiles at different salinities 74
3.3.4.3. Amino acid compositions at different salinities 75
3.4. Discussion 88
3.4.1. Effects of salinity on survival and growth rates of M. sanguinea from larval to adult stage 88
3.4.2. The biochemical composition of M. sanguinea adult body at different salinities 90
Chapter 4. Efficient Removal of Copepods by Physiochemical Treatments in Marphysa sanguinea Culture Systems 96
4.1. Introduction 96
4.2. Materials and methods 99
4.2.1. Exposure interval method for recirculation and flow-through system 99
4.2.1.1. In recirculation system 99
4.2.1.2. In flow-through system 100
4.2.2. Trichlorfon treatment 100
4.2.3. Water analysis methods 100
4.2.4. Data analysis 101
4.3. Results 106
4.3.1. Exposure interval by tidal water drainage 106
4.3.1.1. Number of copepods during experiment in recirculation system without worms 106
4.3.1.2. Number of copepods in recirculation system with broodstock worms 106
4.3.1.3. Number of larval worm in recirculation system with broodstock worms 106
4.3.1.4. Number of copepods in flow-through system 107
4.3.2. Trichlorfon treatment 118
4.3.2.1. Response of larval M. sanguinea to trichlorfon treatment 118
4.3.2.2. Response of copepods to trichlorfon treatment 118
4.3.2.3. Identification of copepods in M. sanguinea rearing system 118
4.4. Discussion 125
Chapter 5. General Discussion and Summary 128
5.1. General Discussion 128
5.2. Summary 131
5.2.1. Larval Production of Marphysa sanguinea under Laboratory Conditions 131
5.2.2. Effects of Salinity on Survival, Growth Rates and Biochemical Compositions on Marphysa sanguinea 131
5.2.3. Efficient Removal of Copepods by Physiochemical Treatments in Marphysa sanguinea Culture Systems 132
Acknowledgments 134
References 136
- Degree
- Doctor
- Files in This Item:
-
-
Download
Effects of Salinity and Copepods on Artificial Seed Production of the Rockworm Marphysa sanguinea (P.pdf
기타 데이터 / 3.96 MB / Adobe PDF
-
Items in Repository are protected by copyright, with all rights reserved, unless otherwise indicated.