Single Molecule Real Time 기술을 이용한 Celeribacter marinus 균주 IMCC12053의 메틸롬 연구 및 Flavobacteriales 목(目) UJ101균주의 비교유전체 연구

Abstract

Single Molecule Real Time (SMRT) technology is one of the Next Generation Sequencing (NGS) tool using Pacific Bioscience (PacBio) RS II Sequencing. This sequencing technology has the advantage of possible methylation analysis. In order to analyze 2 Marine Microorganism (Celeribacter marinus strain IMCC12053, Flavobacteriales bacterium strain UJ101), we used PacBio Sequencing analysis. First, Celeribacter marinus IMCC12053 was isolated from coastal seawater from Yellow Sea of Korea, it was used as the host bacterium for bacteriophage P12053L. We report the complete genome sequence of strain IMCC12053 for further study of the marine bacteriophage P12053L. Genome sequencing data is 3,096,705 base pairs in length by single circular chromosome and the GC content is 56.24%. It contains 3,155 ORFs with 45 tRNAs and 6 rRNAs genes. N6-methyladenosine patterns were also investigated for 32 unmethylated genes and intergenic regions that covered many regulators and phage genes as well as ribosomal RNA genes and tRNA genes. Cryptic N4-methylcytosine pattern was investigated to speculate GpC methylase activity throughout the genome. Comparative genomics with other Celeribacter genomes were carried out for polyaromatic hydrocarbon degradation, but there were no aromatic ring oxygenases in IMCC12053 when compared to Celeribacter indicus P73. Second, Flavobacteriales bacterium strain UJ101was isolated from a xanthid crab shell collected from East Sea of Korea. We report the complete genome sequence of strain UJ101 for the study of metabolic interaction between UJ101 and its host organism. Genome sequencing data is 3,074,209 base pairs in length by single circular chromosome and the GC content was 30.74%. The genome of UJ101 contains 2,698 ORFs with 46 tRNAs and 9 rRNAs genes. According to the annotated list of genes Embden–Meyerhof and pentose phosphate pathway is well conserved, but key enzymes of Entner-Douddoroff pathway were impaired. TCA and glyoxylate cycle were conserved while carbon fixation and one carbon metabolism were mostly lacking except formaldehyde dehydrogenase. UJ101 encodes degradation enzyme including 8 glycosyl transferases, 3 amylases, and 8 peptidases. Biosynthetic enzymes for lysine, tryptophan, phenylalanine, and tyrosine were also impaired. Alcohol and/or organic acid fermentation could not be expected. Strain UJ101 was compared with bacterial genomes isolated from other marine animals (3 strains from invertebrate and 5 strains from fishes). Other related genomes from the same genera were included although they were reportedly isolated from seawater and marine sediments.