PUKYONG

Synthesis of stimuli-responsive drug delivery systems via RAFT polymerization and Diels-Alder click reaction for doxorubicin release

Metadata Downloads
Alternative Title
독소루비신 방출을 위한 RAFT 중합과 Diels-Alder 클릭반응을 이용한 자극감응형 약물전달시스템
Abstract
암세포 표적 기능을 갖는 약물 전달체는 pH변화, 산화-환원 전위 또는 효소등의 내부 환경 변화를 이용하여 치료 효과를 증진 시키거나 심각한 부작용 문제를 극복 할 수 있다.
본 연구에서는 poly(ethylene oxide)-b-poly(furfuryl methacrylate) (PEO-b-PFMA)블록 공중합체의 코어-가교된 산화-환원 반응성 마이셀과 poly(styrene-alt-maleic anhydride)와 다중벽 탄소 나노튜브(MWNT / PSMA-DOX) 기반의 pH 반응성 약물 전달체를 RAFT 중합 및 Diels-Alder 클릭 반응의 장점들을 결합하여 합성하였다.
본 연구를 통해 약물 전달 시스템은 pH 또는 환원제 농도의 변화에 따라 독소루비신을 조절하고 방출 할 수 있는 것으로 확인되었다.
Author(s)
Le Minh Quoc Cuong
Issued Date
2017
Awarded Date
2017. 2
Type
Dissertation
Keyword
RAFT polymerization 약물전달시스템 Diels-Alder 독소루비신
Publisher
부경대학교 대학원
URI
https://repository.pknu.ac.kr:8443/handle/2021.oak/13598
http://pknu.dcollection.net/jsp/common/DcLoOrgPer.jsp?sItemId=000002332585
Affiliation
부경대학교 대학원
Department
대학원 융합디스플레이공학과
Advisor
임권택
Table Of Contents
Chapter 1 1
1.1. Introduction to stimuli-responsive drug delivery system 1
1.1.1. pH response 1
1.1.2. Redox response 2
1.2. Reversible addition-fragmentation chain transfer (RAFT) polymerization 3
1.3. Diels-Alder click reaction 5
1.4. Aim and outline of the thesis 8
1.5. Reference 8
Chapter 2 12
2.1. Introduction 13
2.2. Experimental section 15
2.2.1. Materials 15
2.2.2. Synthesis of PEO-b-PFMA 16
2.2.3. Determination of the critical micelle concentration (CMC) and stability of PEO-b-PFMA micelle 17
2.2.4. DOX loading and CCL micelles of PEO-b-PFMA 17
2.2.5. In vitro drug release 18
2.2.6. Cell viability assay 18
2.2.7. Confocal laser scanning microscopy (CLSM) observation 18
2.2.8. Characterization 18
2.3. Results and discussion 19
2.3.1. Synthesis and characterization of CCL micelles 19
2.3.2. Stability and reactivity of non-CCL and CLL micelles 24
2.3.3. Drug loading 25
2.3.4. In vitro release 25
2.3.5. Cytotoxicity of blank micelles and DOX-loaded micelles 26
2.4. Conclusions 30
2.5. References 30
Chapter 3 34
3.1. Introduction 35
3.2. Experimental section 36
3.2.1. Materials 36
3.2.2. Synthesis of furfuryl bearing RAFT agent (FA-DDMAT) 36
3.2.3. Anchoring RAFT agent on MWNTs 37
3.2.4. Preparation of poly(styrene-alt-maleic anhydride) grafted MWNTs (MWNT/PSMA) by surface initiated ‘‘grafting from’’ polymerization 37
3.2.5. Preparation of MWNT/PSMA-DOX 37
3.2.6. DOX release from MWNT/PSMA-DOX 38
3.2.7. Characterization 38
3.3. Results and discussion 39
3.3.1. Synthesis and characterization 39
3.3.2. In vitro release 43
3.4. Conclusion 44
3.5. References 44
Degree
Master
Appears in Collections:
대학원 > 융합디스플레이공학과
Authorize & License
  • Authorize공개
Files in This Item:

Items in Repository are protected by copyright, with all rights reserved, unless otherwise indicated.