미세조류 형질전환을 위한 디지털 전기천공법의 적용 및 바이오연료 중 불순물 제거 반응기 CFD 모델링

Abstract

For the production of bio-fuel, it is essential to get superior species of microalgae. However, it is hard to get good species in nature. Therefore, the transformation of microalgae by delivering external DNA into cells is very significant. Among various transformation methods, electroporation is known for high efficiency and convenience. However, conventional electroporation system has drawbacks such as high initial cost and low cell viability issue. So in previous study, A digital microfluidic electroporation (EP) method is suggested. In this study, we conduct optimization of parameter effect on digital microfluidic electroporation. Optimization of parameters are separated two categories. One is biological parameter such as cell concentration in cell suspension, type of electroporation medium and temperature of electroporation medium , the other is electrical parameter such as applied voltage and number of pulse in same time. we use fluorescent dye to make a fluorescence ,when attache dsDNA, call ed Yo-Pro-1 to reduce analysis time. we perform the verification the optimization result using plasmid DNA. And superior microalgae use to make a bio-fuel. but bio-fuel include impurities such as water, alcohol, glycerin and catalyst. We performed bio-fuel purification reactor design by computation fluid dynamics simulation. By analyzing the change in flow and impurities concentration at the outlet according to the changes in flow rate, reactor length, and reactor diameter, we have found the minimum catalyst performance for the given flow rate condition and the relation between the reactor performance and the reactor size and shape. We also studied the effects of permeability of the packed bed on the flow and impurities concentration distribution. And then, We analyzed the changes in regeneration process according to purge gas flow rate, catalyst permeability, reactor size, and heat loss of reactor. We have found that the regeneration process is very much affected by temperature changes whereas it is hardly affected by catalyst permeability and porosity. We also estimated the regeneration time according to purge gas flow rate and initial temperatures and have found that increasing purge gas temperature is more effect for fast regeneration. The present work can be utilized to design a purification reactor for a fuel cell.