Isolation of Lactic Acid Bacteria showing Antioxidative and Probiotic Activities from Kimchi and Infant Feces

Abstract

The purpose of this study aimed to investigate the lactic acid bacteria with antioxidative and probiotic activities isolated from Korean healthy infant feces and kimchi. A1, A2, S1, S2, and S3 isolates were assigned to Lactobacillus sp., A3, A4, E1, E2, E3, and E4 isolates were assigned to Leucononstoc sp. on the basis of their physiological properties and 16S ribosomal DNA sequence analysis. Most strains were confirmed as safe bioresources through non-hemolytic activities and non-production of harmful β-glucosidase, β-glucuronidase, tryptophanase, or urease etc. 11 strains showed different resistant to acid (at pH2.5) and bile acids (at concentration of 0.3% oxgall). In addition, 11 strains exhibited antibacterial activity against food-borne bacteria, especially Bacillus cereus, Listeria monocytogenesis, and Escherichia coli. Furthermore, all strains showed the highest levels of hydrophobicity. The results suggest that A1, A3, S1, and S3 have good probiotic activities applicable to the development for functional foods and health-related products. The antioxidant effects of 11 strains culture filtrate include 2,2-diphenyl-1-picryl-hydrazyl (DPPH) radical scavenging capacity, 2.2'-azino-bis (2-ethylbenzthiazoline-6-sulphonic acid) (ABTS) radical cation scavenging activity and superoxide dismutase (SOD)-like activity. DPPH and ABTS radical were measured spectrophotometrically ands uperoxide dismutase(SOD)-like activity was evaluated by measuring the inhibition of pyrogallol autoxidation catalyzed by the superoxide radical. The results revealed that all strains culture filtrate have effectively scavenging activity at 2,2-diphenyl-1-picryl-hydrazyl (DPPH) and 2,2'-azino-bis (3-ethylbenzthiazoline-6sulphonic acid) (ABTS) radicals, and except E1, E2, all strains appeared effectively superoxide dismutas activity. In conclusion, isolated strains A1, A3, S1, and S3 might be contributed to prevent and control several disease associated with oxidative stress using as probiotics.