물리, 열화학적인 전처리 기법을 이용한 꼬시래기로부터 환원당 생산

Abstract

In this study, I tried to produced total reducing sugars from Gracilaria verrucosa using physical or thermochemical pretreatment and subsequent enzymatic hydrolysis. In first section, physical pretreatment with ultra sonication and subsequent enzymatic hydrolysis of G. verrucosa was investigated. As a result, the pretreatment yield was 32.46 ± 0.32% when 2%(w/v) G. verrucosa was treated with 100 mM sulfuric acid solution and 60% of sonicator at room temperature for 30 minutes. The pretreatment and enzyme hydrolysis yield of reducing sugar was 70.67 ± 1.79% and it took 12 hours to complete the enzyme saccharification. It was concluded that the yield of reducing sugar can be obtained with the same significance level when compared with the pre-treatment yield and enzyme hydrolysis yield using a autoclave. HMF, which is an inhibitory substance, was produced more by high pressure sterilization treatment. Sonicator treatment was also able to reduce the completion time of enzymatic hydrolysis of G. verrucosa by up to 45 hours. In second section, sulfamic acid-catalyzed hydrothermal pretreatment and subsequent enzymatic hydrolysis of G. verrucosa was investigated. Best pretreatment condition is 7.5% biomass, 100mM sulfamic acid, 90min, 130℃ at this condition. Total reducing sugar yield is 39.9%. By subsequent enzymatic hydrolysis after pretretment, TRS yield was increased up to 69.1%. These results confirm the usefulness of sulfamic acid in pretreatment of G. verrucosa and the possibility of using sulfamic acid in pretreatment of other seaweeds.