Preparations and characterization of hydroxyapatite-fucoidan nanocomposites for bone tissue engineering

Abstract

Hydroxyapatite (HA) is the promising biomaterial in the construction of artificial bone since last five decades. In the present study, we have developed Hydroxyapatite-Fucoidan (HAF) nanocomposite for bone tissue engineering and subsequently characterized by different analytical techniques for bone graft substitute. The surface morphology and properties of the composite were investigated by fourier transform infrared spectroscopy, X-ray diffraction, thermal gravimetric analysis, scanning electron microscopy, energy dispersive X-ray diffraction and transmission electron microscopy. The result of FT-IR and TGA suggested that presence of fucoidan in HAF nanocomposite. The results of electron microscopy revealed that nanocomposite (HAF) with crystals size 41-153 nm is observed. The biochemical characterization inferred that the prepared nanocomposite were non- toxic and enhanced mineralization effect of HAF was observed which is two times higher than HA. In addition, adipose derived mesenchymal stem cell interaction with HAF was also evaluated, the results shows that no cytoxicity, enhanced mineralization and increased osteogenic markers including type 1 collagen, osteopontin and osteocalcin were observed. Hence, HAF is the promising biomaterial and could be used for bone tissue regeneration.