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치어기 틸라피아와 넙치에 있어서 수산가공부산물을 이용한 사료 첨가제 개발

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Alternative Title
Development of feed additives by using fisheries by-products in juvenile Nile tilapia, Oreochromis niloticus and olive flounder, Paralichthys olivaceus
Abstract
치어기 틸라피아와 넙치에 있어서 수산가공부산물에 이노신산(IMP)첨가와 가공 과정의 pH 조절을 통한 양어사료용 첨가제 개발가능성을 확인하기 위해 수행되었다. 실험 1에서는, 4.9 ± 0.07g (mean ± SD) 인 치어기 틸라피아를 8주간 사육실험하였다. 총 6개의 사료구는 대조구 (CON)와 수산가공부산물 각각 2%의 100% 새우가공부산물 (SSE), 98% SSE + 2% IMP (SSEP2), 96% SSE + 4% IMP (SSEP4), 100% 오징어가공부산물 (SQSE) 그리고 100% 틸라피아가공부산물 (TSE)를 대조구사료 내 대두박과 다른 사료원 2%를 대체하여 제작하였다. 실험 2에서는, 13.4 ± 0.13g (mean ± SD) 인 치어기 넙치를 9주간 사육실험하였다. 총 5개의 사료구는 대조구 (CON)와 수산가공부산물 각각 2%의 100% SSE at pH 2 (SSEL), 100% SSE at pH 4 (SSEH), 95% SSE at pH 2 + 5% IMP (SSELP) 그리고 95% SSE at pH 4 + 5% IMP (SSEHP)를 대조구사료 내 Lysine 과 다른 사료원 2%를 대체하여 2x2 factorial design으로 제작하였다. 사육실험 종료 후, 실험 1에서는 IMP가 첨가되지 않은 SSE 와 4% IMP가 첨가된 SSEP4 의 수산가공부산물 첨가가 치어기 틸라피아에 있어 성장과 면역반응에 효과를 보이는 것으로 판단되었다. 실험 2에서는 가공 과정의 pH 조절에 상관없이 IMP가 첨가되지 않은 SSEL 및 SSEH 와 가공 과정의 pH 가 2와4로 조절 되고 5%IMP가 첨가된 SSELP 및 SSEHP 의 수산가공부산물 첨가가 치어기 넙치에 있어 SSEL 및 SSEH 실험구는 성장에 효과를 보였으며, SSELP 및 SSEHP 실험구는 면역반응에 효과를 보이는 것으로 판단되었다.
Two experiments were conducted to investigate the effects of dietary supplementation of different fisheries by-product extracts with and without inosine momophosphate (IMP) on growth performance and non-specific immune responses in juvenile Nile tilapia and juvenile olive flounder. In the first experiment, juvenile Nile tilapia averaging 4.9 ± 0.07g (mean ± SD) were fed one of the six experimental diets; A basal diet without feed additives was used as control (CON), the other five experimental diets were formulated to include 2% each of 100% shrimp soluble extract (SSE), 98% shrimp soluble extract + 2% IMP (SSEP2), 96% shrimp soluble extract + 4% IMP (SSEP4), 100% squid soluble extract (SQSE) and 100% tilapia soluble extract (TSE) replacing 2% of soybean meal and other ingredients from CON diet. In the second experiment, juvenile olive flounder averaging 13.4 ± 0.13g (mean ± SD) were fed one of the five experimental diets. A basal diet without feed additives was used as control (CON), the other four diets were formulated to include 2% each of 100% SSE processed at low pH (SSEL), 100% SSE processed at high pH (SSEH), 95% SSE + 5% IMP processed in low pH (SSELP) and 95% SSE + 5% IMP processed in high pH (SSEHP) replacing 2% of wheat flour and other ingredients from CON diet. The first experiment’s results that supplementation shrimp soluble extract without (SSE) and with 4% IMP (SSEP4) as feed additives could have beneficial effects on growth performance and immune responses in juvenile Nile tilapia. The second experiment’s results indicated that supplementation of shrimp soluble extract produced in low or high pH without (SSEL and SSEH) and with IMP (SSELP and SSEHP) could have beneficial effects on growth performance by SSEL and SSEH and on immune responses by SSELP and SSEHP in juvenile olive flounder.
Author(s)
송유진
Issued Date
2018
Awarded Date
2018.2
Type
Dissertation
Keyword
수산가공부산물 넙치 틸라피아 사료 첨가제
Publisher
부경대학교
URI
https://repository.pknu.ac.kr:8443/handle/2021.oak/14202
http://pknu.dcollection.net/common/orgView/200000010585
Alternative Author(s)
Yujin Song
Affiliation
부경대학교 대학원
Department
대학원 수산생물학과
Advisor
배승철
Table Of Contents
Abstract ii
List of tables ix
List of figures x
Chapter 1. Introduction 1
Chapter 2. Effects of dietary soluble extracts additive on growth performance and non-specific immune responses in juvenile Nile tilapia, Oreochromis niloticus 4
I. Materials and Methods 4
II. Results 14
III. Discussion 29
Chapter 3. Effects of dietary soluble extracts additive on growth performance and non-specific immune responses in juvenile olive flounder, Paralichthys olivaceus 32
I. Materials and Methods 32
II. Results 41
III. Discussion 56
Acknowledgment 59
References 61
Appendix (raw data) 72
Degree
Master
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대학원 > 수산생물학과
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