형질전환 생산효율 극대화를 위한 세포 배양과 유전체 조작 메커니즘 개선 및 공정 설계 연구

Abstract

For the production of bio-fuel, many researches have been actively conducted on discovering and improving the excellent microalgae species capable of high-speed growth and high lipid content. In many cases, exogenous materials delivery is highly dependent on the cell cycle status and growth curve. Commonly, The highest expression following electroporation is obtained when cells are in mid-log phase growth. That’s why cell wall is weak in mid-log phase. So, In this phase, It facilitates maximum exogenous DNA uptake. However, It takes many time to prepare cells in mid-log phase. So, It is good to find ways to reduce time of cell preparation. The way to reduce time of cell preparation is to increase initial concentration. So, It is possible to save time of cell preparation and obtain higher exogenous materials delivery efficiency. And for more than a decade, conventional benchtop EP systems using cuvette type unit cell has been widely used. However, current conventional EP systems require high equipment cost because of using high voltage and it is suffered from side effects due to an electrochemical reaction, local pH variations, Joule heating, and gas bubble formation near electrodes. Recently, a unique digital microfluidic EP system based on droplet contact charging phenomenon was suggested as an alternative solution. The productivity achieved by the proposed digital microfluidic EP system was comparable to that of conventional benchtop EP system but it is still insufficient in some biological experiments. Here, we demonstrated a high throughput EP system capable of dealing with billions of cells more effectively for more efficient transformation research. So this paper can be useful for biological researcher to do electroporation.