엄나무 추출물의 첨가가 닭발 육수의 품질에 미치는 영향
- Alternative Title
- Effect Adding Extracts of Kalopanacis cortex on Quality Characteristics of Chicken Feet Stock
- Abstract
- The scrapped natural resources can be the most efficient food materials. In this study, we aim to use the chicken feet, discarded food materials to make seasonable stock. For this, we investigated the effects of hot water soluble of Kalopanacis cortex (WEK) on the chicken stock. We established the optimum ratio of water and K. cortex for the conditions of hot water soluble extracts having antioxidant activity. In an analysis of compositions of WEK, the content of carbohydrate, ash and lipid was 12.3%, 2.6% and 1.1% respectively. In the minerals, the potassium content was 185.2 mg/100g
Which was the highest level among minerals. In an analysis of free amino acids, the p-serine content was the highest with the level of 41.36 mg/100g. However, in the chicken feet stock, the taurine level was the highest among all recovered amino acids with the level of 5.90 mg/100g. In the composition of unsaturated fatty acids, the linoleic acid level was highest and it was 44.1% in the chicken feet stock. In the compositions of free sugar and organic acid in WEK, the contents of glucose and cirtric acid were highest and it was 2,046.5 mg/100g and 87.7 mg/100g, respectively. In the chicken feet stock, the content of collagen, cholesterol and hypoxanthine was 311.50, 2.0 and 5.81 mg/100g, respectively.
The extracting conditions for the highest polyphenol compound from WEK was 15 h at 95℃ after cold treatment. With the increasis of time and temperature, the superoxide dismutase activity (SOD) of WEK was increased. The total polyphenol compound content of WEK extracted at 95℃ for 15h after cold treatment was 612 μg/mL, which was higher than other extraction condition. The DPPH(1, 1-diphenyl-2-picrylhydraxyl) radical scavenger activity of WEK extracted at 95℃ for 15h after cold treatment was the highest as 78.8%, which was comparable with ascorbic acid showing 70.2% of DPPH activity. The hydroxyl radical scavenger activity(HRSA) of WEK was the highest as 69.0% at 95℃ for 15 h after cold treatment, and it was stronger than ascorbic acid showing 56.6%. These results elucidated that the optimum extracting conditions of WEK having antioxidant activities, which may provide valuable information for using K. cortex as the source of functional food. Additionaly, 12 g of K. cortex in 500 g of chicken feet was effective to remove bloody ordor and to enhance taste of chicken feet stock
In analyzing of volatile compounds used gas chromatographic mass spectrometer (GC-MS). the contents of alcohols and aldehydes were highest in the WEK, while, the contents of aldehyde were the higehest in the WEK, while the contents of aldehydes and alkanes were highest in the chicken feet stock. Interestingly, the contents of alcohols and alkanes were highest in the mixed stock of chicken feet stock and WEK (5:5). Although n-hexanal, heptanal, n-decanal, 2-nonenal, or nonanal which causes bloody odor were detected in the chicken feet stock and WEK.
In conclusion, our study shows that the content of polyphenol, and the activities of DPPH, HRAS, and SOD-like are high in water extraction of K.cortex at the temperature ranges of 55~95℃. In addition, we found that the off-flavor in the chicken feet stock was dramatically reduced in the mixed stock of chicken feet and WEK (5:5 ratio). In a GC-MS analysis, the content of aldehydes was 50.4% in the chicken feet stock, but it was reduced to 15.9% in the mixed stock of WEK. These results suggests that the antioxidant activities of WEK may inhabit oxidation of lipid, thus the formation of Strecker aldehyde and eventually lead to mask the Maillard reaction. Therefore, the antioxidant activies of WEK may be critical to off-flavor of the chicken feet stock, which could be applicable in the field of food chemistry and food processing.
- Author(s)
- Hoon-Suk Yang
- Issued Date
- 2014
- Awarded Date
- 2014. 2
- Type
- Dissertation
- Publisher
- 부경대학교
- URI
- https://repository.pknu.ac.kr:8443/handle/2021.oak/1568
http://pknu.dcollection.net/jsp/common/DcLoOrgPer.jsp?sItemId=000001966977
- Affiliation
- Graduate School
- Department
- 대학원 식품공학과
- Advisor
- 조영제
- Table Of Contents
- 목차
Abstract……………………………………………………………i
목차…………………………………………………………………v
표 목록……………………………………………………….viii
그림 목록…………………………………………………………x
서론 ………………………………………………………………..1
재료 및 방법…………………………………………………….. 8
1. 실험재료.........................................................................8
2. 실험방법..........................................................................9
2.1 총 폴리페놀 함량…………………………………………………12
2.2 DPPH radical scavenging activity 측정 ……………………..12
2.3 Hydroxyl radical scavenging activity 측정 ……………13
2.4 SOD-like activity 측정 …………………………………………14
2.5 일반성분 분석 ……………………………………………………..15
2.6 무기질 분석 ………………………………………………………..15
2.7 유리 아미노산 분석 ………………………………………………16
2.8 지방산 분석 ………………………………………………………16
2.9 유리당 및 유기산 분석 …………………………………………17
2.10 콜라겐 함량 ………………………………………………..18
2.11 콜레스테롤 함량 ……………………………………………..19
2.12 핵산관련물질 함량 …………………………………………….20
3. 휘발성 성분 분석……………………………………………………21
4.관능평가……………………………………………………………25
5.통계처리……………………………………………………………26
결과 및 고찰
I.추출 조건에 따른 해동피의 열수추출물의 항산화효과
………………………………………………………………………….27
1. 총 폴리페놀 화합물의 함량……………………………………… 27
2. DPPH radical 소거 활성능……………………………………30
3. HRSA radical 소거 활성능…………………………………40
4. SOD 유사 활성능……………………………………………………47
II. 일반성분…………………………………………………………….54
1. 해동피와 닭발 육수의 일반성분…………………………………54
2. 무기질 함량………………………………………………………57
3. 유리 아미노산 조성…………………………………………………59
4. 지방산 조성…………………………………………………63
5. 유리당과 유기산 조성…………………………………………..66
6. Collagen, cholesterol, 핵산관련 물질의 조성………………….68
7. 관능 검사…………………………………………………………70
III. 해동피 열수 추출물의 첨가에 따른 닭발 육수의 이취 저감
……………………………………………………………………………72
1. 닭발 육수의 휘발성 성분 분석……………………………72
2. 해동피 열수 추출물의 휘발성 성분 분석………………….78
3. 혼합육수의 휘발성 분석…………………………………84
4. 휘발성 성분에 대한 고찰……………………………………..85
5. 해동피 열수 추출물의 이취 저감 효과……………………89
5.1. 지방산 산화억제 효과…………………………………………89
5.2. Maillard reaction masking 억제 효과……………………91
요 약………………………………………………………………..99
감사의 글 ………………………………………………………………103
참고문헌……………………………………………………….106
- Degree
- Doctor
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- 대학원 > 식품공학과
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