Sulfadiazine의 경구투여에 따른 흰다리새우에서의 약물동태학 및 독성학적 특성 연구

Abstract

This study was conducted to investigate the pharmacokinetic and toxicological properties of sulfadiazine when administered orally to whiteleg shrimp. Feeds containing 80, 240 and 400 mg/kg of sulfadiazine were administered orally at 20℃ and 28℃ for 7 days. After oral administration of sulfadiazine, the amount of drug residue in the tissue (muscle, hemolymph, and hepatopancreas) was analyzed over time using LC-MS/MS. Pharmacokinetic characteristics were derived from the drug residual data using Pk Solver program. Residual amount decreased with time in muscle, hemolymph, and hepatopancreas, and the residual amount increased significantly as the concentration of sulfadiazine increased. In the muscle, hemolymph, and hepatopancreas administered sulfadiazine, the time to reach days of maximum concentration (Tmax) was 0 days at all concentrations, and the maximum concentration (Cmax) was in the order of hemolymph, muscle, and hepatopancreas. In all dose concentration groups, a large amount of sulfadiazine was excreted between 0 and 1 days after the end of the administration, and the residual concentration became less than 0.1 mg/kg on the 3rd day after the end of the administration. The release rate of sulfadiazine was more rapidly at 28 ℃ than at 20 ℃. Biochemical analysis of hemolymph and histopathologic analysis through H&E staining were performed to confirm the toxicity of sulfadiazine to whiteleg shrimp. As a result of biochemical index analysis, the number of hemocytes did not show any significant difference at all concentrations compared to the control group. GOT and total protein were significantly decreased in all concentration groups compared to the control group. GPT, total cholesterol and glucose showed a tendency to decrease in the 80mg/kg concentration group compared to the control group, and rather increase in the high concentration group. As a result of evaluating histopathologic toxicity to muscle, hepatic pancreas, and antennal glands, there was no change in all drug-administered groups compared to the control group. In conclusion, the results of this study on the pharmacokinetic and toxicity evaluation of sulfadiazine can be used as basic data necessary to establish the maximum residue level (MRL) and withdrawal period of sulfadiazine applicable to crustaceans in the future.