Trichlorfon 노출에 따른 뱀장어(Anguilla japonica)의 체내 잔류량 및 독성 연구 수행

Abstract

In this study, pharmacokinetic analysis was performed according to trichlorfon (TCF) exposure to obtain the data necessary for the establishment of maximum residue level (MRL) for eels, and the effect of TCF on drug metabolism and physiological function of eels was also investigated. First, pharmacokinetic parameters of TCF were obtained in muscle, liver, and blood using the PK Solver program based on the results of measuring the drug residual concentration in the body according to the bath administration of TCF. In blood, muscle and liver, Cmax is 25.8698-357.421, 129.905-1043.73, 40.4662-375.198, Tmax is 0.1278-1.31581h, 1.18943-3.338h, 0.1361-5.39822h, and half-life(t1/2) is 2.12919-3.92153h, 5.29793-10.3544h, and 0.65284-13.8079h, respectively. Second, the expression of the drug metabolism enzyme CYP450 gene was analyzed. TCF mainly expressed CYP 1 family genes in each tissue. AjCYP1A gene was mainly expressed in the heart and liver, AjCYP1B gene was mainly expressed in the brain, eye, and heart. On the other hand, AjCYP1C1 gene was expressed without differences between tissues. Third, superoxide dismutase (SOD), catalase (CAT), glutathione-S-transferase (GST), acetylcholinesterase (AChE) and Cortisol activities were measured. The activities of antioxidant enzymes (GST, SOD and CAT) were maintained at a steady state without significant change, and AChE activity in the brain was significantly inhibited and recovered again. plasma cortisol levels, a stress index, increased as time elapsed after TCF administration, showing the highest levels between 30 minutes and 1 hour. After that, it began to decrease and returned to its original state. Taken together, the results of this study indicate that TCF not only affects the drug metabolism process in eel, but also inhibits AChE activity, resulting in neurotoxicity.