근적외선 제어형 키토산 기반 하이드로겔의 합성 및 약물 전달 시스템으로의 응용

Abstract

Chitosan is one of the most widely used materials to fabricate hydrogels. It has low toxicity, immune-stimulatory activities and excellent biocompatibility. In addition to these advantages, a biodegradable and near-infrared (NIR) light-responsive hydrogel is one of the most promising strategies as a on demand drug releasing carrier. In this study, Biodegradable and NIR light-triggered chitosan-based hydrogels were prepared as drug delivery carriers for antibiotic drug ciprofloxacin (CIP). The ex situ-forming hydrogel that have covalently bonded cross-linked networks fabricated readily via inverse electron demand Diels-Alder (iEDDA) click chemistry between norbornene-functionalized chitosan (Cs-Nb-COOH) and methyl-tetrazine cross-linkage containing diselenide bonds (Se-MeTz). Cross-linking densities were multipleized into three conditions to manipulate their properties. NIR sensitive indocyanine green (ICG) and a drug, CIP were post-loaded into the dried hydrogel matrix in phosphorate-buffered saline (PBS) at three different pH values (3.7, 5.2, and 7.4). The release profile for the hydrogels was examined in three different PBS media same as drug loading. The results indicated that the release profile has pH-dependent influences and rapid release of CIP under NIR light. ICG generated reactive oxygen species (ROS) which could cleavege diselenide bonds in the polymer matrix, inducing release of entrapped CIP.