Mitochondrial Genomes of Cynoglossus senegalensis and Chrysichthys nigrodigitatus from the coastal water of Cameroon
- Alternative Title
- 카메룬 연근해에 서식하는 Cynoglossus senegalensis와 Chrysichthys nigrodigitatus의 전장 미토콘드리아게놈의특성규명
- Abstract
- The complete mitogenomes of Cynoglossus senegalensis and Chrysichthys nigrodigitatus were determined by the combination of high-throughput sequencing and the traditional PCR-based cloning. The size of each complete mitochondrial genome was 16,519 bp for C. senegalensis and 16,514bp for C. nigrodigitatus, respectively. Translocation of putative control region (D-Loop) and a tRNA gene in the mitochondrial genome occurred and their location was identified between ND1 and tRNA-Gln genes. Phylogenetic analysis showed that C. senegalensis was most closely related to Cynoglossus sinicus and Cynoglossus bilineatus, which were geographically distant and further study should be conducted to understand the complicated evolution of fish in Cynoglossidae. Different from C. senegalensis, the mitogenome of C. nigrodigitatus showed a canonical mitochondrial genome organization in which 13 Protein-coding genes, 22 tRNA genes, 2 rRNA genes, and 2 noncoding regions including the light-strand replication origin (OL) and a putative control region (CR) were conserved. All tRNA genes were predicted to fold into the typical cloverleaf secondary structures with the typical base-pairing except for tRNA-Ser (AGC). The phylogenetic analysis with currently known complete mitogenome sequences in Siluriformes showed that C. nigrodigitatus is most closely related to Auchenoglanis occidentalis forming a family Claroteidae cluster.
Keywords: Mitochondrial genome, Cynoglossus senegalensis, Chrysichthys nigrodigitatus, Cameroon,
- Issued Date
- 2019
- Awarded Date
- 2019. 2
- Type
- Dissertation
- Publisher
- 부경대학교
- Affiliation
- 부경대학교 글로벌수산대학원
- Department
- 글로벌수산대학원 국제수산과학협동과정
- Advisor
- 박원규
- Table Of Contents
- 1.Introduction 1
2.Materials and Methods 7
2.1. Study Site: Geographical Location 7
2.1.1. Sample collection and Preservation 9
2.2. Genomic DNA extraction 9
2.2.1. PCR amplification and sequencing 11
2.2.2. Assembly of mitochondrial genome by the bioinformatics analysis 12
3.Results and Discussion 13
3.1. Morphological and molecular Identification of Cynoglossus senegalensis and Chrysichthys nigrodigitatus. 13
3.2. Complete mitochondrial genome of Cynoglossus senegalensis. 16
3.3. Organization of full length mitochondrial genome of Cynoglossus senegalensis 19
3.4. Structure of tRNA genes of Cynoglossus senegalensis 22
3.5. Phylogenetic tree of Cynoglosus senegalensis 24
3.6. The complete mitochondrial genome of Chrysichthys nigrodigitatus 27
3.7. Organization of full length mitochondrial genome of Chrysichthys nigrodigitatus. 29
3.8. Structure of tRNA genes of Chrysichthys nigrodigitatus. 31
3.9. Phylogenetic tree of Chrysichthys nigrodigitatus 33
4.Conclusion 36
5.Acknowledgements 37
6.References 38
List of Figures
Figure 1. Map of Cameroon Coastline (Gabche and Hockey, 1995) 8
Figure 2. Cynoglossus senegalensis (A) and Chrysichthys nigrodigtatus (B) collected from the Coastal Waters of Cameroon. 15
Figure 3. Mitochondrial genomic organization of Cynoglossus senegalensis 18
Figure 4. Putative secondary structure of 22 tRNA genes in the mitochondrial genome of Cynoglossus senegalensis 23
Figure 5. Phylogenetic tree of Cynoglossus senegalensis 26
Figure 6. Mitochondrial genomic organization of Chrysichthys nigrodigitatus 28
Figure 7. Putative secondary structure of 22 tRNA genes in Mitochondrial genome of Chrysichthys nigrodigitatus 32
Figure 8. Phylogenetic tree of Chrysichthys nigrodigitatus within Order Siluriformes 35
List of Tables
Table 1. List of Species specific primers used during this study 10
Table 2. Organization of the full-length mitochondrial genome of Cynoglossus senegalensis 21
Table 3. Organization of the mitochondrial genome of Chrysichthys nigrodigitatus 30
- Degree
- Master
- Appears in Collections:
- 글로벌수산대학원 > 국제수산과학협동과정
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