차세대 서열 분석을 통한 해삼 Apostichopus japonicus의 장내 미생물체 분석 연구

Abstract

All animals live in complex interactions with microorganisms and the intestine is the most important organ where such interaction occurs. Understanding the intestinal microbial diversity may be essential to understand the animal. In this study, we investigated the intestinal microbial diversity of the sea cucumber Apostichopus japonicus, which is an important economical marine invertebrate in the Asian coasts. We randomly selected nine sea cucumbers at four different sampling times and the intestine was divided into anterior and posterior parts and used for DNA extraction. The 16S rRNA gene amplicon sequencing approach using tagged PCR with the Illumina MiSeq platform, was used to obtain the sequences from each sample. A total of 1,614,748 optimized reads and 350,254 operational taxonomic units (OTUs) were obtained from a total of 72 intestines from 36 sea cucumber samples. The sequences in the anterior intestines and posterior intestines were assigned to 42 and 35 phyla, respectively. The results showed that major phyla are Proteobacteria, Bacteroidetes, Verrucomicrobia, and Planctomycetes. The community profiles became similar in the posterior intestine. The taxonomical classification profiles and the Unifrac PCoA chart showed the effect of sampling times on the bacterial communities in the different intestinal regions.