PUKYONG

GUIDE OAK 국가리포지터리 부경대학교

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Alternative Title: 우간다 빅토리아 호수에서 수집한 바이오 매스로 3 개의 효모와 Kariba weed (Salvinia molesta)를 사용한 초음파 처리로 바이오 에탄올 발효 강화

Abstract: Kariba weed (Salvinia molesta) was used as biomass for ethanol production by separate hydrolysis and fermentation (SHF). Monosaccharides from Kariba weed hydrolysate was produced using thermal acid hydrolysis, sonication and enzymatic saccharification. The optimal conditions for thermal acid hydrolysis of 12% (w/v) Kariba weed slurry were evaluated as 200 mM HNO3 at 121°C for 60 min yielding 10.2 g/L monosaccharides. Sonication for 45 min before enzymatic saccharification, yielded more monosaccharides of 18.7 g/L. Enzymatic saccharification with 16 U/mL Cellic CTec2 produced 35.4 g/L monosaccharides. Fermentation was performed with Saccharomyces cerevisiae, Kluyveromyces marxianus or Pichia stipitis with sonicated Kariba weed hydrolysate. The control fermentations were carried out using Kariba weed hydrolysate without sonication. The improvement of ethanol production from sonicated Kariba weed hydrolysate using P. stipitis produced 15.9 g/L ethanol with ethanol yield coefficient, YEtOH = 0.45, K. marxianus produced 14.7 g/L ethanol with YEtOH = 0.41. S. cerevisiae produced the lowest yield of 13.2 g/L ethanol with YEtOH = 0.37 as it utilized only glucose not xylose. Sonication of Kariba weed is essential in the ethanol production to enhance the productivity of monosaccharides. P. stipitis is the best yeast using hydrolysates with the mixture of glucose and xylose to produce ethanol.

URI: https://repository.pknu.ac.kr:8443/handle/2021.oak/23715
http://pknu.dcollection.net/common/orgView/200000287354

Table Of Contents: 1.Introduction 1
2.Materials and methods 4
2.1.Raw materials 4
2.2.Thermal acid hydrolysis 4
2.3.Sonication of Kariba weed 5
2.4.Optimization of Enzymatic Saccharification 6
2.5.Selection of yeast for fermentation 7
2.5.1.Seed Culture 7
2.6.Ethanol Fermentation 7
2.7.Analytical Method and Statistical analyses 8
3.Results and Discussion 10
3.1.Composition of Kariba weed 10
3.2.Optimization of pretreatment methods 11
3.2.1.Thermal acid hydrolysis 11
3.2.2.Sonication of Kariba weed 14
3.3.Enzymatic Saccharification 16
3.4.Fermentation 19
3.4.1.Fermentation using S.cerevisiae 19
3.4.2.Fermentation using K.marxianus 21
3.4.3.Fermentation using P.stipitis 21
4.Process Simulation of ethanol production from Kariba weed 25
5.Conclusion 28
References 30
Acknowledgements 37

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