Molecular characterization of The mitochondrial genome of Silurus soldatovi from Nakdong River, Republic of Korea

Abstract

The entire mitochondrial genome structure of Soldatov’s catfish, Silurus soldatovi has been characterised. The samples were gathered from a tributary of River Nakdong, South Korea. The specimens were subjected to bioinformatics assembly concerning next generation sequencing (NGS) declaims to establish mitogenome. The intact circular mitogenome of S. soldatovi was 16525 base pairs or bps long and determined 13 canonical protein-coding genes, 2 rRNAs as well as 22 tRNAs. Further, the mitogenome encompasses 2 non-coding regions, a control region designated as D-loop (890 bp long), an origin locus for light strand replication designated as OL (31 bp long). These regions were well conserved in the mitogenome of S. soldatovi. Additionally, the A+T content was found to be 56.20 percent while the G+C content was a little less and found to be 43.80 percent. The complete gene sequence of mitogenome of S. soldatovi was found to be matching with other Silurids. As many as 28 genes were positioned on the H strand, while the remaining nine genes were located on the L strand. Excluding COX1 gene with the initiation codon GTG, 12 protein-coding genes were anticipated to have ATG as the start codon. When compared to other presently identified mitogenome sequences of the family Siluridae, Silurus soldatovi exhibited the greatest similarity of 99.38 percent with Chinese haplotype S. soldatovi (NC022723) and of 97.17 percent with Chinese haplotype Silurus asotus (JX087351).