미세조류의 형질전환을 위한 디지털 전기천공시스템 개발 및 외인성 물질 전달의 최적화 연구

Abstract

In this study, we developed a digital electroporation system using a 3D printer and compared the delivery efficiency of exogenous materials (fluorescent dye, DNA, RNA) using a digital electroporation system, and confirmed the conditions of electroporation for which the transmission efficiency is optimal. The FDM and SLA methods were used as 3D printing methods and the surface properties of the materials were compared using surface images and contact angles. FDM materials are generally used after the post-treatment, so they were experimented on the post-treatment specimens. According to the results of the contact angle measurement and SEM image, the FDM method material is hydrophobic and has a smooth surface. However, even with transparent materials, it is difficult to ensure the transparency of the print results. The SLA method revealed small protrusions on the surface with SEM images. According to the results of the contact angle measurements and SEM images, the SLA method material is hydrophilic. Sufficient transparency was confirmed when using transparent materials. The fabrication of the electroporation chip of the digital electroporation system and the demonstration in the actual experimental environment confirmed the possibility of digital electroporation system development using 3D printer. The delivery efficiency was compared using exogenous materials such as fluorescent dyes (Yo-Pro-1), DNA and RNA in a digital electroporation system. The efficiency of delivery depends on the type of exogenous material used for delivery, which is related to the particle size and properties of the extraneous material. Through this, it was confirmed that the optimal electroporation conditions vary depending on the type of exogenous material used in the transformation experiment using the digital electroporation system. Through this study, we compared the surface characteristics of 3D printer materials and confirmed the possibility of developing digital electroporation system using 3D printer in the actual experimental environment. In addition, digital electroporation conditions that optimize delivery efficiency were verified by comparing the delivery efficiency of exogenous materials.