식이성 간비대 유발 넙치 (Paralichthys olivaceus)에 대한 혈액성상 및 상처회복에 관한 연구

Abstract

The liver is an organ responsible for the processing, storage, transportation, and detoxification of metabolic intermediates in vivo. Decreased liver function can cause many clinical problems. Liver hypertrophy is likely to cause hypoxic damage in hepatic cells, resulting in mechanical distortion and obstruction of surrounding sinusoidal capillary and Disse space. If structural abnormality in the liver occurs, it will cause functional impairment on the basis of structure function relationship, which will result in a condition that is vulnerable to secondary infections or external stimuli. In fish, identifying and managing the health status of the liver is very important and it is thought that there is a need to study how histological changes in the liver affect the fish. Therefore, in this experiment, we wanted to make sure that the high fat diet could artificially change the histological structure. At the same time, we observed the process of wounds healing as part of an effort to see if the abnormality of liver induced by the high fat diet was negatively related to the health of the fish. After feeding 2%/fish body weight of the feed containing 24% lipid in olive flounder for 2 week, three wounds on back with a 2mm biopunch. After the wounds were cut, the samples were sampled on the 1, 7, 14 and 21 days to observed a change in the wound and blood component for 3 weeks. As a result, A liver hypertrophy appeared in the 2 weeks after fed and was formed in most fish in the 4 weeks. The Total cholesterol was significantly high in the 3, 5weeks in groups(HF, HF-injury). As a result of comparing the recovery of the wounds between liver hypertrophy and normal, there was no significant difference. The thickness of the epithelial cells showed no difference between the two groups(HFI, FI) and the wound recover process in HFI and FI groups was shown in the order of re-epitheliaization, inflammation and remodeling, and the cell components and processes that appeared in each process were the same. There was no difference in wound healing process between 2 groups, and so a state of liver hypertrophy which is a pre-stage of fatty change does not seem significantly to affect the healthiness of fish.