키토산올리고당-금나노입자의 특성 및 Pseudomonas aeruginosa PAO1에 의해 생성된 바이오필름 억제

Abstract

Being opportunistic pathogen and development of multiple antimicrobial resistance properties, there is an alternative demand for the combating Pseudomonas aeruginosa infection. Chitosan is one of alternative biologically derived product that has been proven to be exhibits diverse biological activities. However, the low water-solubility of chitosan limits its biological activity and the chemical modification, resulting in the study on shortening the polymer size as alternate possibilities to maintain its activity. Furthermore, nanotechnology is one of the emergence approaches for the enhanced biological activity of any potential drug for treating the pathogenic infection. Several reports showed that gold nanoparticle will be as a potential candidate for the treating bacterial infection. In the present study it synthesized a potential chitooligosaccharides-gold nanoparticle by taking chitooligosaccharides and chloroauric acid materials. The synthesized chitooligosaccharides-gold nanoparticle has been characterized by UV-visible spectroscopy, Fourier transform infrared spectroscopy, field emission electron microscopy and dynamic light scattering. It was observed the treatment of chitooligosaccharides-gold nanoparticle to P. aeruginosa resulting in a concentration dependent biofilm inhibition and dispersion of virulence factors. Furthermore, it showed the suppression of swimming and twitching motility by the treatment of nanoparticle, whereas it enhanced the swarming motility. Findings from this study provide important insights to the potentials of chitooligosaccharides-gold nanoparticles as a new effective drug for prevention and treatment of P. aeruginosa biofilm-related infections.