국내 해삼(Apostichopus japonicus)의 집단유전학분석과 혈연관계 조사 연구

Abstract

Marine biological resources are economic resources as an important component of natural ecosystems. In addition, The genetic diversity of living things creates various values in the ecosystem. Marine biological resources are managed worldwide for diversity management and conservation. In 1992, the Convention on Biological Diversity (CBD) was signed and has the right to biological resources. Recently, although the Access to genetic resources and Benefit-Sharing (ABS) agreement was signed, most of the studies related to the ecological characteristics, habitat, and seed production of marine organisms are conducted, and there are few studies for population structure and characteristics and kinship analysis. In this study, genetic diversity and population structure analysis, and kinship analysis were conducted on sea cucumbers. Genetic diversity and population structure analysis were performed using the developed 13 Microsatellite marker for sea cucumber. As a result of analyzing the genetic diversity of sea cucumbers by region in Korea, the average number of alleles was highest in KJ(13.92). The observed heterozygosity(Ho) was highest in YY(0.660). The FIS was the lowest in the YY(0.104) and the highest in the KS(0.231). The high inbreeding coefficients are likely to genetic decline and rapid change in the genetic characteristics. In the genetic distance analysis, YY was found to be relatively far from GJ(0.101) and KJ(0.099). The Fst was 0.006 to 0.034 in all groups, which is within the low level (0.000 to 0.050) suggested by Wright (1978). In the STRUCTURE analysis, It was divided into the YY and other groups(KJ, GJ, WD, DH, KS). This result was similar to that of genetic distance and PCoA. PIC and Exclusion power were calculated using the genotyping results of 943 sea cucumbers. The Power of discrimination was 6.886X10-16, the Power of exclusion was 3.468X10-10, and the Power of sibling discrimination was 5.21X10-06. And we calculated false-positive and false-negative error rates. In the parent/child, the relationship could be identified in likelihood ratio ‘1’. In sibling relationships, the relationship could be identified in likelihood ratio of‘10’. Taken together, this study can be used as basic data on releasing effect and genetic management for conservation by confirming the genetic diversity, current status, and genetic structure of sea cucumbers designated as endangered by IUCN.